Sonic Hedgehog Protein (SHH) (AA 24-197) (His tag)

Produktnummer ABIN2181760

Produktdetails

Target: Sonic Hedgehog (SHH)

Protein-Typ: Recombinant

Proteineigenschaft: AA 24-197

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Sonic Hedgehog Protein ist gelabelt mit His tag.

Sequenz: AA 24-197

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.4 kDa. The protein migrates as 22 kDa under reducing (R) condition (SDS-PAGE).

Reinheit: >95 % as determined by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

Referenzen

Kaushal, Popli, Sankhwar, Shukla, Dwivedi: "Sonic hedgehog protects endometrial hyperplasial cells against oxidative stress via suppressing mitochondrial fission protein dynamin-like GTPase (Drp1)." in: Free radical biology & medicine, Vol. 129, pp. 582-599, (2018) (PubMed).

Antigendetails

Target: Sonic Hedgehog (SHH)

Andere Bezeichnung: Sonic Hedgehog (SHH Produkte)

Hintergrund: Sonic hedgehog protein (SHH) is also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

Molekulargewicht: 20.4 kDa

NCBI Accession: NP_000184

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development