FAS Protein (AA 26-173) (Fc Tag)

Produktnummer ABIN2181070

Produktdetails

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 26-173

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses FAS Protein ist gelabelt mit Fc Tag.

Sequenz: AA 26-173

Produktmerkmale: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 42.8 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Reinheit: >95 % as determined by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM Glycine, pH 7.5

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

Antigendetails

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Andere Bezeichnung: Fas (FAS Produkte)

Hintergrund: The Fas is also known as FAS receptor (FasR), apoptosis antigen 1 (APO-1 or APT), cluster of differentiation 95 (CD95) or tumor necrosis factor receptor superfamily member 6 (TNFRSF6). is a death receptor on the surface of cells that leads to programmed cell death (apoptosis). It is one of two apoptosis pathways, the other being the mitochondrial pathway. FasR is located on chromosome 10 in humans and 19 in mice. Similar sequences related by evolution (orthologs) are found in most mammals. Fas forms the death-inducing signaling complex (DISC) upon ligand binding. Membrane-anchored Fas ligand trimer on the surface of an adjacent cell causes trimerization of Fas receptor. This event is also mimicked by binding of an agonistic Fas antibody, though some evidence suggests that the apoptotic signal induced by the antibody is unreliable in the study of Fas signaling. To this end, several clever ways of trimerizing the antibody for in vitro research have been employed.Upon ensuing death domain (DD) aggregation, the receptor complex is internalized via the cellular endosomal machinery. This allows the adaptor molecule FADD to bind the death domain of Fas through its own death domain. Recently, Fas has also been shown to promote tumor growth, since during tumor progression, it is frequently downregulated or cells are rendered apoptosis resistant. Cancer cells in general, regardless of their Fas apoptosis sensitivity, depend on constitutive activity of Fas. This is stimulated by cancer-produced Fas ligand for optimal growth.

Molekulargewicht: 42.8 kDa

Pathways: p53 Signalweg, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity