Tumor Necrosis Factor (TNF) (Soluble), (AA 80-235) (Active) Protein

Details zu Produkt Nr. ABIN2007701, Anbieter: Anmelden zum Anzeigen
Proteinname
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Proteineigenschaft
Soluble, AA 80-235
28
12
12
8
6
5
5
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Spezies
Maus
115
49
29
9
6
5
5
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Quelle
Escherichia coli (E. coli)
155
26
16
6
4
3
3
2
2
2
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The recombinant mouse TNF-α consists of 157 amino acids and migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the soluble form of mouse TNF-α (NP_038721.1) (Leu 80-Leu 235) was expressed, with an initial Met at the C-terminus.
Predicted N-Term: Met
Reinheit > 98 % as determined by SDS-PAGE
ProductDetails: Biological Activity Comment Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
Hintergrund Synonyms: DIF,TNF-a,TNF-alpha,Tnfa,TNFalpha,Tnfsf1a,TNFSF2
Molekulargewicht 17 kDa
NCBI Accession NP_038721
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile 50 mM Tris 0.2M NaCl, pH 7.2
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Tumor Necrosis Factor (TNF) (Soluble), (AA 80-235) (Active) protein (ABIN2007701) Tumor Necrosis Factor (TNF) (Soluble), (AA 80-235) (Active) protein
Produkt verwendet in: Sieber, Lange, Kollmorgen, Erhardt, Quaas, Gontarewicz, Sass, Tiegs, Kreienkamp: "Sharpin contributes to TNF? dependent NF?B activation and anti-apoptotic signalling in hepatocytes." in: PLoS ONE, Vol. 7, Issue 1, pp. e29993, 2012 (PubMed).

Allgemeine Veröffentlichungen Sheng, Hu, Ni, Rowen, Lokensgard, Peterson: "TNF-alpha-induced chemokine production and apoptosis in human neural precursor cells." in: Journal of leukocyte biology, Vol. 78, Issue 6, pp. 1233-41, 2005 (PubMed).

Chen, Goeddel: "TNF-R1 signaling: a beautiful pathway." in: Science (New York, N.Y.), Vol. 296, Issue 5573, pp. 1634-5, 2002 (PubMed).

Locksley, Killeen, Lenardo: "The TNF and TNF receptor superfamilies: integrating mammalian biology." in: Cell, Vol. 104, Issue 4, pp. 487-501, 2001 (PubMed).

Guo, Dunbar, Yang, Pfeffer, Donner: "Induction of Jak/STAT signaling by activation of the type 1 TNF receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 6, pp. 2742-50, 1998 (PubMed).

Black, Rauch, Kozlosky, Peschon, Slack, Wolfson, Castner, Stocking, Reddy, Srinivasan, Nelson, Boiani, Schooley, Gerhart, Davis, Fitzner, Johnson, Paxton, March, Cerretti: "A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells." in: Nature, Vol. 385, Issue 6618, pp. 729-33, 1997 (PubMed).

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