Coagulation Factor II (thrombin) (F2) (full length), (AA 1-618) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2007695, Anbieter: Anmelden zum Anzeigen
Proteinname
  • zgc:66319
  • wu:fb57c10
  • wu:fd42e09
  • wu:fd59d01
  • Cf-2
  • Cf2
  • FII
  • thrombin
  • PT
  • RPRGL2
  • THPH1
  • prothrombin
  • AA959438
  • AI255193
  • AI327365
  • C1sa
  • HTRA
  • HTRA1
  • F2
  • coagulation factor II (thrombin)
  • coagulation factor II, thrombin
  • cell division cycle 34
  • chymase 1
  • coagulation factor II
  • complement component 1, s subcomponent 1
  • HtrA serine peptidase 1
  • HtrA serine peptidase 2
  • melanoma-associated antigen B17
  • f2
  • F2
  • Cdc34
  • CMA1
  • C1s1
  • Htra1
  • HTRA2
  • LOC783975
  • HTRA1
Proteineigenschaft
full length, AA 1-618
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Spezies
Maus
16
10
10
6
3
Quelle
Human Cells
15
6
5
4
3
2
2
2
2
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses Prothrombin Protein ist gelabelt mit His tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The secreted recombinant mouse F2 consists of 605 amino acids (pro form) and has a calculated molecular mass of 69.3 kDa. The apparent molecular mass of the recombinant mouse F2 is approximately 85 kDa in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the full length of mouse F2 (NP_034298.1) (Met 1-Gly 618) was expressed, with a C-terminal polyhistidine tag.
Predicted N-Term: Gln 25
Reinheit > 96 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC R&D Systems, Catalog # ES011.
The specific activity is > 2000 pmoles/min/μg.
(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
Hintergrund Synonyms: Cf-2,Cf2,FII
Molekulargewicht 69.3 kDa, 85 kDa
NCBI Accession NP_034298
Pathways Komplementsystem, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Coagulation Factor II (thrombin) (F2) (full length), (AA 1-618) (Active) protein (His tag) (ABIN2007695) Coagulation Factor II (thrombin) (F2) (full length), (AA 1-618) (Active) protein (His tag)
Allgemeine Veröffentlichungen Li, Adams, Nangalia, Esmon, Huntington: "Molecular basis of thrombin recognition by protein C inhibitor revealed by the 1.6-A structure of the heparin-bridged complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 12, pp. 4661-6, 2008

Li, Adams, Nangalia, Esmon, Huntington: "Molecular basis of thrombin recognition by protein C inhibitor revealed by the 1.6-A structure of the heparin-bridged complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 12, pp. 4661-6, 2008 (PubMed).

Fife, Mader, Stone, Brill, Satterfield, Norfleet, Zwernemann, Ryaby, Carney: "Thrombin peptide Chrysalin stimulates healing of diabetic foot ulcers in a placebo-controlled phase I/II study." in: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, Vol. 15, Issue 1, pp. 23-34, 2007 (PubMed).

Degen, McDowell, Sparks, Scharrer: "Prothrombin Frankfurt: a dysfunctional prothrombin characterized by substitution of Glu-466 by Ala." in: Thrombosis and haemostasis, Vol. 73, Issue 2, pp. 203-9, 1995 (PubMed).

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