Interleukin 25 (IL25) (AA 17-169), (Mature) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2007396, Anbieter: Anmelden zum Anzeigen
Proteinname
  • IL25
  • Il25
  • Ly6elg
  • IL17E
  • IL-17e
  • Il17e
  • RGD1561632
  • interleukin 25
  • myeloid derived growth factor
  • IL25
  • Mydgf
  • Il25
Proteineigenschaft
AA 17-169, Mature
8
3
2
2
2
1
1
1
1
1
1
Spezies
Maus
30
17
12
Quelle
Human Cells
40
4
3
2
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses IL25 Protein ist gelabelt mit His tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Produktmerkmale The secreted recombinant mouse IL25 consists of 175 amino acids and has a calculated molecular mass of 20 kDa. As a result of glycosylation, rmIL25 migrates with the MW of approximately 30-35 kDa in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the mature form of mouse IL25 (NP_542767.1) (Val 17-Ala 169) was expressed with a N-terminal polyhistidine tag.
Predicted N-Term: Gly
Reinheit > 80 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment 1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL25-His at 10 μg/mL (100 μl/well) can bind biotinylated mouse IL17BR-His with a linear range of 3.125-50 ng/mL.
2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED50 for this effect is 4-14ng/mL.
Hintergrund Synonyms: IL-17e,Il17e
Molekulargewicht 20 kDa, 30-35 kDa
NCBI Accession NP_542767
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Interleukin 25 (IL25) (AA 17-169), (Mature) (Active) protein (His tag) (ABIN2007396) Interleukin 25 (IL25) (AA 17-169), (Mature) (Active) protein (His tag)
Allgemeine Veröffentlichungen Angkasekwinai, Park, Wang, Wang, Chang, Corry, Liu, Zhu, Dong: "Interleukin 25 promotes the initiation of proallergic type 2 responses." in: The Journal of experimental medicine, Vol. 204, Issue 7, pp. 1509-17, 2007

Angkasekwinai, Park, Wang, Wang, Chang, Corry, Liu, Zhu, Dong: "Interleukin 25 promotes the initiation of proallergic type 2 responses." in: The Journal of experimental medicine, Vol. 204, Issue 7, pp. 1509-17, 2007 (PubMed).

Tamachi, Maezawa, Ikeda, Iwamoto, Nakajima: "Interleukin 25 in allergic airway inflammation." in: International archives of allergy and immunology, Vol. 140 Suppl 1, pp. 59-62, 2006 (PubMed).

Ikeda, Nakajima, Suzuki, Kagami, Hirose, Suto, Saito, Iwamoto: "Mast cells produce interleukin-25 upon Fc epsilon RI-mediated activation." in: Blood, Vol. 101, Issue 9, pp. 3594-6, 2003 (PubMed).

Büning, Genschel, Weltrich, Lochs, Schmidt: "The interleukin-25 gene located in the inflammatory bowel disease (IBD) 4 region: no association with inflammatory bowel disease." in: European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics, Vol. 30, Issue 5, pp. 329-33, 2003 (PubMed).