EPH Receptor A2 (EPHA2) (AA 585-976) (Active) protein (GST tag,His tag)

Details zu Produkt Nr. ABIN2006006, Anbieter: Anmelden zum Anzeigen
Proteinname
  • EPHA2
  • epha2
  • MGC89505
  • ARCC2
  • CTPA
  • CTPP1
  • CTRCT6
  • ECK
  • AW545284
  • Eck
  • Myk2
  • Sek-2
  • Sek2
  • EPH receptor A2
  • EphA2
  • epoxide hydrolase
  • Eph receptor A2
  • EPHA2
  • epha2
  • ephA2
  • Epha2
Proteineigenschaft
AA 585-976
3
1
1
1
1
1
1
1
1
1
1
1
1
1
Spezies
Human
12
4
Quelle
Baculovirus infected Insect Cells
6
2
2
2
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses EPH Receptor A2 Protein ist gelabelt mit GST tag,His tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
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Produktmerkmale The recombinant human EPHA2 /GST chimera consists of 629 amino acids and has a calculated molecular mass of 72.1 kDa. The recombinant protein migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human EPHA2 (P29317) (Leu585-Ile976) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Predicted N-Term: Met
Reinheit > 95 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment The specific activity was determined to be 50 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
Hintergrund Synonyms: ARCC2,CTPA,CTPP1,CTRCT6,ECK
Molekulargewicht 72.1 kDa, 62 kDa
UniProt P29317
Forschungsgebiet Signaling
Pathways RTK Signalweg
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Supplied as sterile 20 mM Tris, 500 mM NaCl, 3 mM DTT, pH 8.5, 10 % gly
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Haltbarkeit 12 months
Bilder des Herstellers
 image for EPH Receptor A2 (EPHA2) (AA 585-976) (Active) protein (GST tag,His tag) (ABIN2006006) EPH Receptor A2 (EPHA2) (AA 585-976) (Active) protein (GST tag,His tag)
Allgemeine Veröffentlichungen Zhang, Njauw, Park, Naruse, Asano, Tsao: "EphA2 is an essential mediator of UV radiation-induced apoptosis." in: Cancer research, Vol. 68, Issue 6, pp. 1691-6, 2008 (PubMed).

Rikova, Guo, Zeng, Possemato, Yu, Haack, Nardone, Lee, Reeves, Li, Hu, Tan, Stokes, Sullivan, Mitchell, Wetzel, Macneill, Ren, Yuan, Bakalarski, Villen, Kornhauser, Smith, Li, Zhou, Gygi, Gu et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. ..." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Nowakowski, Cronin, McRee, Knuth, Nelson, Pavletich, Rogers, Sang, Scheibe, Swanson, Thompson: "Structures of the cancer-related Aurora-A, FAK, and EphA2 protein kinases from nanovolume crystallography." in: Structure (London, England : 1993), Vol. 10, Issue 12, pp. 1659-67, 2002 (PubMed).