Met Proto-Oncogene (MET) (Extracellular Domain), (AA 1-932) (Active) protein (Fc Tag,His tag)

Details zu Produkt Nr. ABIN2003239, Anbieter: Anmelden zum Anzeigen
Proteinname
  • AUTS9
  • HGFR
  • RCCP2
  • c-Met
  • AI838057
  • HGF
  • Par4
  • Hgfr
  • c-met
  • MET proto-oncogene, receptor tyrosine kinase
  • met proto-oncogene
  • MET
  • Met
Proteineigenschaft
Extracellular Domain, AA 1-932
29
13
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Spezies
Human
47
5
5
3
1
Quelle
Human Cells
21
17
10
8
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses MET Protein ist gelabelt mit Fc Tag,His tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The mature recombinant human c-Met/Fc is a disulfide-linked tetramer composed of two proteolytically cleaved α and β subunits. Each α and β together with the C-terminal Fc tag consists of 1155 amino acids and has a predicted molecular mass of 129.5 (α =32.5 + Fc tagged β=97) kDa. The rh c-MET/Fc heterodimer thus migrates with apparent molecular mass of approximately 45 kDa and 120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.

Protein Structure: A DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human c-Met (NP_000236) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Predicted N-Term: Glu 25
Reinheit > 95 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 1 μg/ml (100ul/well) can bind Human c-MET / HGFR with a linear range of 0.31-160ng/ml. Scatchard analysis showed the affinity constant (Kd) of human HGF bound to human c-MET / HGFR was 0.52 nM .
Hintergrund Synonyms: AUTS9,c-Met,DFNB97,HGFR,RCCP2
Molekulargewicht 45 kDa, 120 kDa
NCBI Accession NP_000236
Pathways RTK Signalweg, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Met Proto-Oncogene (MET) (Extracellular Domain), (AA 1-932) (Active) protein (Fc Tag,His tag) (ABIN2003239) Met Proto-Oncogene (MET) (Extracellular Domain), (AA 1-932) (Active) protein (Fc Tag,His tag)
Allgemeine Veröffentlichungen Boccaccio, Comoglio: "Invasive growth: a MET-driven genetic programme for cancer and stem cells." in: Nature reviews. Cancer, Vol. 6, Issue 8, pp. 637-45, 2006

Boccaccio, Comoglio: "Invasive growth: a MET-driven genetic programme for cancer and stem cells." in: Nature reviews. Cancer, Vol. 6, Issue 8, pp. 637-45, 2006 (PubMed).

Birchmeier, Birchmeier, Gherardi, Vande Woude: "Met, metastasis, motility and more." in: Nature reviews. Molecular cell biology, Vol. 4, Issue 12, pp. 915-25, 2003 (PubMed).

Pennacchietti, Michieli, Galluzzo, Mazzone, Giordano, Comoglio: "Hypoxia promotes invasive growth by transcriptional activation of the met protooncogene." in: Cancer cell, Vol. 3, Issue 4, pp. 347-61, 2003 (PubMed).

Weidner, Di Cesare, Sachs, Brinkmann, Behrens, Birchmeier: "Interaction between Gab1 and the c-Met receptor tyrosine kinase is responsible for epithelial morphogenesis." in: Nature, Vol. 384, Issue 6605, pp. 173-6, 1996 (PubMed).

Bottaro, Rubin, Faletto, Chan, Kmiecik, Vande Woude, Aaronson: "Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product." in: Science (New York, N.Y.), Vol. 251, Issue 4995, pp. 802-4, 1991 (PubMed).

Stephenson, Tronick, Aaronson: "Isolation from BALB/c mouse cells of a structural polypeptide of a third endogenous type C virus." in: Cell, Vol. 3, Issue 4, pp. 347-53, 1975 (PubMed).

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