Complement Component 5 (C5) (AA 679-751), (active) (Active) Protein

Details zu Produkt Nr. ABIN2003124, Anbieter: Anmelden zum Anzeigen
Proteinname
  • C5
  • LOC565774
  • C5a
  • C5b
  • CPAMD4
  • Hc
  • RGD1561905
  • He
  • complement C5
  • complement component 5
  • hemolytic complement
  • C5
  • c5
  • Hc
Proteineigenschaft
AA 679-751, active
3
2
2
1
1
1
1
1
1
1
1
1
1
Spezies
Human
7
6
2
1
1
Quelle
Escherichia coli (E. coli)
8
4
3
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The recombinant human C5a consists of 74 amino acids and has a predicted molecular mass of 8.3 kDa which is also estimated by SDS-PAGE.

Protein Structure: A DNA sequence encoding the active form of human C5a (Leu 679-Arg 751) was expressed.
Predicted N-Term: Based on N-terminal sequencing analysis, the first 7 aa of C5a is: MLQKKIE
Reinheit > 94 % as determined by SDS-PAGE
ProductDetails: Biological Activity Comment Measured by its ability to induce N-acetyl-β-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is typically 5-15 ng/ml.
Hintergrund Synonyms: C5a,C5b,C5D,C5Da,Complement 5a,CPAMD4,ECLZB
Molekulargewicht 8.3 kDa
Forschungsgebiet Innate Immunity, Complement Factors
Pathways Komplementsystem, Carbohydrate Homeostasis
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Complement Component 5 (C5) (AA 679-751), (active) (Active) protein (ABIN2003124) Complement Component 5 (C5) (AA 679-751), (active) (Active) protein
Produkt verwendet in: Seow, Lim, Iyer, Suen, Ariffin, Hohenhaus, Sweet, Fairlie: "Inflammatory responses induced by lipopolysaccharide are amplified in primary human monocytes but suppressed in macrophages by complement protein C5a." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 8, pp. 4308-16, 2013 (PubMed).

Allgemeine Veröffentlichungen Riedemann, Guo, Gao, Sun, Hoesel, Hollmann, Wetsel, Zetoune, Ward: "Regulatory role of C5a on macrophage migration inhibitory factor release from neutrophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 2, pp. 1355-9, 2004

Riedemann, Guo, Gao, Sun, Hoesel, Hollmann, Wetsel, Zetoune, Ward: "Regulatory role of C5a on macrophage migration inhibitory factor release from neutrophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 2, pp. 1355-9, 2004 (PubMed).

Huber-Lang, Sarma, McGuire, Lu, Padgaonkar, Younkin, Guo, Weber, Zuiderweg, Zetoune, Ward: "Structure-function relationships of human C5a and C5aR." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6115-24, 2003 (PubMed).

Sumichika, Sakata, Sato, Takeshita, Ishibuchi, Nakamura, Kamahori, Ehara, Itoh, Ohtsuka, Ohbora, Mishina, Komatsu, Naka: "Identification of a potent and orally active non-peptide C5a receptor antagonist." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49403-7, 2002 (PubMed).

Gerard, Gerard: "C5A anaphylatoxin and its seven transmembrane-segment receptor." in: Annual review of immunology, Vol. 12, pp. 775-808, 1994 (PubMed).

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