Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) (Active) protein (His tag)

Details zu Produkt Nr. ABIN1981682, Anbieter: Anmelden zum Anzeigen
Proteinname
  • PGKB
  • PGKPS
  • dJ417L20.2
  • Pgk-2
  • Tcp-2
  • PGK2
  • phosphoglycerate kinase 2
  • PGK2
  • Pgk2
  • cbbK
Proteineigenschaft
AA 1-417
6
2
1
1
1
Spezies
Human
7
1
1
1
1
Quelle
Escherichia coli (E. coli)
3
2
2
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
His tag
Applikation
SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Sequenz MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
Produktmerkmale Molecule Information: 46.9 kDa (437 aa, 1-417 aa + His Tag)
Reinheit ≥85 % by SDS-PAGE
Biological Activity Comment Specific activity is >11 units/mg. One unit will convert 1.0 μmole of beta-NADH per minute in a coupled system with GAPDH at pH 8.0 at 25°C
Unit Definition One unit will convert 1.0 μmole of beta-NADH per minute in a coupled system with GAPDH at pH 8.0 at 25°C
Hintergrund PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternative Names: Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS
Molekulargewicht 46.9 kDa
Gen-ID 5232
UniProt P07205
Forschungsgebiet Signaling, Metabolism
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/mL
Buffer 0.5 mg/mL solution in 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1 M NaCl and 1 mM DTT
Konservierungsmittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Centrifuge the vial prior to opening.
Lagerung -20 °C
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) (Active) protein (His tag) (ABIN1981682) Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) (Active) protein (His tag)
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