TIMP Metallopeptidase Inhibitor 1 (TIMP1) (Active) protein (His tag)

Details zu Produkt Nr. ABIN1981492, Anbieter: Anmelden zum Anzeigen
Proteinname
  • TIMP-1
  • TIMP1
  • DKFZp468A0912
  • CLGI
  • EPA
  • EPO
  • HCI
  • TIMP
  • Timp
  • Clgi
  • TIMP metallopeptidase inhibitor 1
  • tissue inhibitor of metalloproteinase 1
  • TIMP1
  • Timp1
  • LOC100009047
Spezies
Human
32
6
3
2
2
2
2
1
1
1
1
Quelle
HEK-293 Cells
22
8
5
5
5
4
4
1
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses TIMP1 Protein ist gelabelt mit His tag.
Applikation
SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke CellExp™
Produktmerkmale Molecule Information: This protein contains C-terminal polyhistidine tag and has a calculated MW of 24 kDa. DTT-reduced protein migrates as 28-32 kDa polypeptide in SDS-PAGE.
Reinheit ≥95 % by SDS-PAGE
Sterilität 0.22 μm filtered
Endotoxin-Niveau <1 EU/μg by LAL method
ProductDetails: Biological Activity Comment Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.The IC50 value is approximately 2.5 nM.
Hintergrund TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein a member of the TIMP family. There are four members of the family, TIMP1, TIMP2, TIMP3 and TIMP4. TIMP1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.

Alternative Names: TIMP1, TIMP-1, CLGI, EPA, EPO, FLJ90373, HCI, TIMP, TIMP metallopeptidase inhibitor 1
Gen-ID 7076
UniProt P01033
Forschungsgebiet Proteolysis / Ubiquitin, Angiogenesis, Extracellular Matrix, Matrix Metalloproteinases, Cancer, Inflammation
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Human CellExp™ Recombinant Proteins provide tremendous benefits for scientists performing human cell based studies:
- They contain all the post-translational modifications (which affects the activity, function and half-life) seen in human proteins.
- They mimic native human proteins in terms of sub-unit assembly, folding, secretion and other processes.
- They show the same chemical and biological properties like stability, isoelectric point, solubility, binding affinities and biological activity.
- They are produced in serum-free medium; hence they exhibit minimal batch to batch variability and are pathogen free products.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8 °C for up to 1 month. For extended storage, it is recommended to store at -20 °C.
Buffer Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Handhabung Centrifuge the vial prior to opening.
Lagerung -20 °C
Informationen zur Lagerung This solution can be stored at 2-8 °C for up to 1 month. For extended storage, it is recommended to store at -20 °C.
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for TIMP Metallopeptidase Inhibitor 1 (TIMP1) (Active) protein (His tag) (ABIN1981492) TIMP Metallopeptidase Inhibitor 1 (TIMP1) (Active) protein (His tag)
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