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EPO Protein

EPO Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant ≥ 95 % Func Active
Produktnummer ABIN1305139
  • Target Alle EPO Proteine anzeigen
    EPO (Erythropoietin (EPO))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Spezies
    • 15
    • 8
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 18
    • 15
    • 7
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Applikation
    Functional Studies (Func)
    Marke
    BD Pharmingen™
    Spezifität
    Biological Activity Measured using TF-1 indicator cells
    Specific Activity: 0.1 - 1.0 x 10^8 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation).
    ED50: 0.1 - 1.0 ng/ml
    Observed linear dose response range: >100 fold
    Produktmerkmale
    Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, and containing 1 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant mouse EPO is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law.
    Aufreinigung
    The recombinant mouse EPO is > 95 % pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law.
    Reinheit
    ≥ 95 %
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    The endotoxin level is ≤ 0.1 ng per µg of mouse EPO, as measured in a chromogenic LAL assay.
    Top Product
    Discover our top product EPO Protein
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    BD Pharmingen™ Mouse Erythropoietin Recombinant Protein - Reactivity Ms

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.1 mg/mL
    Buffer
    Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, and containing 1 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.
    Konservierungsmittel
    Azide free
    Handhabung
    Rapidly thaw and quick-spin product prior to use. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
  • Nagao, Suga, Okano, Masuda, Narita, Ikura, Sasaki: "Nucleotide sequence of rat erythropoietin." in: Biochimica et biophysica acta, Vol. 1171, Issue 1, pp. 99-102, (1992) (PubMed).

    Krantz: "Erythropoietin." in: Blood, Vol. 77, Issue 3, pp. 419-34, (1991) (PubMed).

    Kitamura, Tange, Terasawa, Chiba, Kuwaki, Miyagawa, Piao, Miyazono, Urabe, Takaku: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, (1989) (PubMed).

    McDonald, Lin, Goldwasser: "Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene." in: Molecular and cellular biology, Vol. 6, Issue 3, pp. 842-8, (1986) (PubMed).

    Shoemaker, Mitsock: "Murine erythropoietin gene: cloning, expression, and human gene homology." in: Molecular and cellular biology, Vol. 6, Issue 3, pp. 849-58, (1986) (PubMed).

    JACOBSON, GOLDWASSER, FRIED, PLZAK: "Role of the kidney in erythropoiesis." in: Nature, Vol. 179, Issue 4560, pp. 633-4, (1957) (PubMed).

  • Target
    EPO (Erythropoietin (EPO))
    Andere Bezeichnung
    EPO (EPO Produkte)
    Synonyme
    EPO Protein, EP Protein, MVCD2 Protein, erythropoietin Protein, erythropoietin S homeolog Protein, erythropoietin a Protein, EPO Protein, epo Protein, epo.S Protein, Epo Protein, epoa Protein
    Substanzklasse
    Hormone
    Hintergrund
    Mouse erythropoietin (EPO) is a 30 kD heavily glycosylated protein containing 166 amino acids. The carbohydrate residues compose approximately 30 % of the molecule by weight.3 It shares 80 % and 95 % homology with human and rat EPO, respectively. EPO functions as the survival and proliferation factors of late erythroid progenitor cells (CFU-E). In adult mammals, EPO is synthesized almost exclusively in the kidneys.
    Pathways
    JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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