Coagulation Factor III (thromboplastin, Tissue Factor) (F3) protein (His tag)

Details zu Produkt Nr. ABIN1098373, Anbieter: Anmelden zum Anzeigen
Proteinname
  • f3
  • tf
  • AA409063
  • CD142
  • Cf-3
  • Cf3
  • TF
  • TFA
  • coagulation factor IIIa
  • tissue factor
  • coagulation factor III
  • coagulation factor III (thromboplastin, tissue factor)
  • f3a
  • tf
  • F3
Spezies
Human
20
5
5
3
3
1
Quelle
Escherichia coli (E. coli)
20
5
4
2
2
2
1
1
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
His tag
Applikation
SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Reinigung purified by chromatography
Reinheit > 90 % by SDS - PAGE
Hintergrund Tissue factor, also known as F3 or CD142, is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Recombinant human F3 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Molekulargewicht 25.9 kDa (228aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
NCBI Accession NP_001984
Forschungsgebiet Cardiovascular
Pathways Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
Kommentare

Synonyms: Tissue factor, CD142, TF, TFA

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/ml (determined by Bradford assay)
Buffer 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM DTT
Lagerung 4 °C
Informationen zur Lagerung Avoid repeated freezing and thawing cycles.
Bilder des Herstellers
 image for Coagulation Factor III (thromboplastin, Tissue Factor) (F3) protein (His tag) (ABIN1098373) Coagulation Factor III (thromboplastin, Tissue Factor) (F3) protein (His tag)
Allgemeine Veröffentlichungen Bogdanov, Balasubramanian, Hathcock, Vele, Lieb, Nemerson: "Alternatively spliced human tissue factor: a circulating, soluble, thrombogenic protein." in: Nature medicine, Vol. 9, Issue 4, pp. 458-62, 2003 (PubMed).

Mackman, Morrissey, Fowler, Edgington: "Complete sequence of the human tissue factor gene, a highly regulated cellular receptor that initiates the coagulation protease cascade." in: Biochemistry, Vol. 28, Issue 4, pp. 1755-62, 1989 (PubMed).

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