Annexin V Protein (Alexa Fluor 488)

Produktnummer ABIN1047953

Produktdetails

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Protein-Typ: Recombinant

Spezies: Maus

Wirt: Bitte anfragen

Aufreinigungstag / Konjugat: Dieses Annexin V Protein ist gelabelt mit Alexa Fluor 488.

Produktmerkmale: Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
Gene Name: Anxa5

Benötigtes Material: Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2
Propidium iodide: 50 g/ml, final concentration 1 g/ml

Anwendungsinformationen

Applikationshinweise: 5 l are suitable for one assay

Testdurchführung:

Staining protocol:
1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
3. Incubate for 15min at room temperature in the dark
4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen

Vermes, Haanen, Steffens-Nakken, Reutelingsperger: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).

Antigendetails

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Andere Bezeichnung: Annexin V-Alexa488 (ANXA5 Produkte)

Synonyme: anx Protein, anx5 Protein, ANX V Protein, anxa5 Protein, cb989 Protein, wu:fa98f06 Protein, wu:fj10f10 Protein, MGC89158 Protein, ANX5 Protein, ENX2 Protein, PP4 Protein, RPRGL3 Protein, Anx5 Protein, R74653 Protein, LC5 Protein, enx2 Protein, annexin A5 Protein, annexin A5b Protein, Annexin A5 Protein, annexin A5 L homeolog Protein, ANXA5 Protein, anxa5b Protein, anxa5 Protein, Anxa5 Protein, anxa5.L Protein

Gen-ID: 11747

UniProt: P48036

Pathways: Apoptose