EIF1 Protein (AA 1-113, full length) (GST tag)

Produktnummer ABIN1046987

Produktdetails

Target: EIF1 (Eukaryotic Translation Initiation Factor 1 (EIF1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-113, full length

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses EIF1 Protein ist gelabelt mit GST tag.

Applikation: ELISA

Sequenz: MSAIQNLHSF DPFADASKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLVEI GLAKDDQLKV HGF

Produktmerkmale: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Reinheit: 90 %

Anwendungsinformationen

Kommentare:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handhabung: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Referenzen

Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, (2004) (PubMed).

Mendell, Medghalchi, Lake, Noensie, Dietz: "Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes." in: Molecular and cellular biology, Vol. 20, Issue 23, pp. 8944-57, (2000) (PubMed).

Sheikh, Fernandez-Salas, Yu, Hussain, Dinman, Peltz, Huang, Fornace: "Cloning and characterization of a human genotoxic and endoplasmic reticulum stress-inducible cDNA that encodes translation initiation factor 1(eIF1(A121/SUI1))." in: The Journal of biological chemistry, Vol. 274, Issue 23, pp. 16487-93, (1999) (PubMed).

Fields, Adams: "Expressed sequence tags identify a human isolog of the suil translation initiation factor." in: Biochemical and biophysical research communications, Vol. 198, Issue 1, pp. 288-91, (1994) (PubMed).

Antigendetails

Target: EIF1 (Eukaryotic Translation Initiation Factor 1 (EIF1))

Andere Bezeichnung: Eukaryotic translation initiation factor 1 protein (EIF1 Produkte)

Synonyme: A121 Protein, EIF-1 Protein, EIF1A Protein, ISO1 Protein, SUI1 Protein, Sui1-rs1 Protein, eukaryotic translation initiation factor 1 Protein, EIF1 Protein, Eif1 Protein

Hintergrund: Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.

Molekulargewicht: 40.1 kD

UniProt: P41567