Caspase 1 Protein (CASP1) (AA 120-269, partial) (GST tag)

Produktnummer ABIN1046767

Produktdetails

Target: Caspase 1 (CASP1)

Protein-Typ: Recombinant

Proteineigenschaft: AA 120-269, partial

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses Caspase 1 Protein ist gelabelt mit GST tag.

Applikation: ELISA

Sequenz: NPAMPTSSGS EGNVKLCSLE EAQRIWKQKS AEIYPIMDKS SRTRLALIIC NEEFDSIPRR TGAEVDITGM TMLLQNLGYS VDVKKNLTAS DMTTELEAFA HRPEHKTSDS TFLVFMSHGI REGICGKKHS EQVPDILQLN AIFNMLNTKN

Produktmerkmale: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Reinheit: 90 %

Anwendungsinformationen

Kommentare:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handhabung: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Referenzen

Miller, Ayala, Egger, Raju, Yamin, Ding, Gaffney, Howard, Palyha, Rolando: "Purification and characterization of active human interleukin-1 beta-converting enzyme from THP.1 monocytic cells." in: The Journal of biological chemistry, Vol. 268, Issue 24, pp. 18062-9, (1993) (PubMed).

Thornberry, Bull, Calaycay, Chapman, Howard, Kostura, Miller, Molineaux, Weidner, Aunins: "A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes." in: Nature, Vol. 356, Issue 6372, pp. 768-74, (1992) (PubMed).

Cerretti, Kozlosky, Mosley, Nelson, Van Ness, Greenstreet, March, Kronheim, Druck, Cannizzaro: "Molecular cloning of the interleukin-1 beta converting enzyme." in: Science (New York, N.Y.), Vol. 256, Issue 5053, pp. 97-100, (1992) (PubMed).

Antigendetails

Target: Caspase 1 (CASP1)

Andere Bezeichnung: Caspase-1 protein (CASP1 Produkte)

Synonyme: ICE Protein, IL1BC Protein, P45 Protein, Il1bc Protein, Ice Protein, CASP1 Protein, CG5370 Protein, DCP-1 Protein, DCP1 Protein, Dcp1 Protein, Dmel\\CG5370 Protein, ccp1 Protein, dcp-1 Protein, dcp1 Protein, l(2)01862 Protein, l(2)02132 Protein, caspase 1 Protein, caspase 1, apoptosis-related cysteine peptidase Protein, caspase 4, apoptosis-related cysteine peptidase Protein, Death caspase-1 Protein, caspase-1 Protein, caspase Protein, caspase recruitment domain-containing protein 16 Protein, caspase 4 Protein, CASP1 Protein, Casp1 Protein, CASP4 Protein, Dcp-1 Protein, Caspase1 Protein, LOC5564196 Protein, LOC451519 Protein

Hintergrund: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.

Molekulargewicht: 44.2 kD

UniProt: P29466

Pathways: Apoptose, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome