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FK506 Binding Protein 2, 13kDa (FKBP2) (AA 22-142) Peptid

FKBP2 Reaktivität: Human Wirt: Escherichia coli (E. coli) SDS Dithiothreitol (DTT)
Produktnummer ABIN935096
  • Target Alle FKBP2 Produkte
    FKBP2 (FK506 Binding Protein 2, 13kDa (FKBP2))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Protein Region
    AA 22-142
    Spezies
    Human
    Quelle
    • 2
    Escherichia coli (E. coli)
    Applikation
    SDS-PAGE (SDS)
    Sequenz
    MATGAEGKRK LQIGVKKRVD HCPIKSRKGD VLHMHYTGKL EDGTEFDSSL PQNQPFVFSL GTGQVIKGWD QGLLGMCEGE KRKLVIPSEL GYGERGAPPK IPGGATLVFE VELLKIERRT EL
    Produktmerkmale
    Purified recombinant Human FKBP2 protein
    Expression System: E.coli
    Bioactivity: Specific activity is > 150 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH8.0 using chymotrypsin.
    Reinheit
    > 90 % pure
  • Applikationshinweise
    FKBP2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    Testdurchführung
    1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
      2. Add recombinant FKBP2 protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
      3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
      4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
      5. Record the increase in A410 nm for 20 minutes
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Supplied as a liquid in 20 mM Tris buffer, pH 8.0, containing 10 % glycerol and 0 mM DTT.
    Konservierungsmittel
    Dithiothreitol (DTT)
    Vorsichtsmaßnahmen
    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    RT/-20 °C
    Informationen zur Lagerung
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target
    FKBP2 (FK506 Binding Protein 2, 13kDa (FKBP2))
    Synonyme
    FKBP-13 Peptide, PPIase Peptide, FKBP12 Peptide, Fkbp2 Peptide, 13kDa Peptide, FKBP-2 Peptide, mFKBP13 Peptide, mFKBP2 Peptide, RGD1560660 Peptide, zgc:101826 Peptide, FK506 binding protein 2 Peptide, FK506 binding protein 1a Peptide, FK506 binding protein 2 L homeolog Peptide, FKBP2 Peptide, Fkbp1a Peptide, Fkbp2 Peptide, fkbp2 Peptide, fkbp2.L Peptide
    Hintergrund
    FKBP2 is peptidyl proline isomerase enzyme and a member of the immunophilin protein family. This protein is predominantly expressed in thymus and T cells and plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. FKBP2 is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. Recombinant FKBP2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: FKBP 13 protein, FK506 binding protein 2 13kDa protein, FKBP 2, FKBP-2, FKBP13 protein, EC 5.2.1.8 protein, FKBP-13 protein, PPIase protein, Peptidyl prolyl cis trans isomerase protein, FKBP-2 protein, Rotamase., Peptidyl-prolyl cis-trans isomerase FKBP2 13 kDa FKBP protein, FK506 binding protein 2 (13kD) protein, Rapamycin binding protein protein, Proline isomerase protein, FKBP2, FKBP 2 protein, FKBP 2 protein
    Molekulargewicht
    13.4 kDa (122 AA)
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