FK506 Binding Protein 2, 13kDa (FKBP2) (AA 22-142) Peptid

Details zu Produkt Nr. ABIN935096, Anbieter: Anmelden zum Anzeigen
Proteinname
  • 13kDa
  • FKBP-2
  • mFKBP13
  • mFKBP2
  • RGD1560660
  • zgc:101826
  • FKBP-13
  • PPIase
  • FK506 binding protein 2, 13kDa
  • FK506 binding protein 2
  • FKBP2
  • Fkbp2
  • fkbp2
Protein Region
AA 22-142
Spezies
Human
Quelle
Escherichia coli (E. coli)
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
SDS-PAGE (SDS)
Optionen
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Sequenz MATGAEGKRK LQIGVKKRVD HCPIKSRKGD VLHMHYTGKL EDGTEFDSSL PQNQPFVFSL GTGQVIKGWD QGLLGMCEGE KRKLVIPSEL GYGERGAPPK IPGGATLVFE VELLKIERRT EL
Produktmerkmale Purified recombinant Human FKBP2 protein
Expression System: E.coli
Bioactivity: Specific activity is > 150 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH8.0 using chymotrypsin.
Reinheit > 90 % pure
Hintergrund FKBP2 is peptidyl proline isomerase enzyme and a member of the immunophilin protein family. This protein is predominantly expressed in thymus and T cells and plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. FKBP2 is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. Recombinant FKBP2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: FKBP 13 protein, FK506 binding protein 2 13kDa protein, FKBP 2, FKBP-2, FKBP13 protein, EC 5.2.1.8 protein, FKBP-13 protein, PPIase protein, Peptidyl prolyl cis trans isomerase protein, FKBP-2 protein, Rotamase., Peptidyl-prolyl cis-trans isomerase FKBP2 13 kDa FKBP protein, FK506 binding protein 2 (13kD) protein, Rapamycin binding protein protein, Proline isomerase protein, FKBP2, FKBP 2 protein, FKBP 2 protein
Molekulargewicht 13.4 kDa (122 AA)
Forschungsgebiet Signaling, Protein Modifications, Stress Response
Applikationshinweise FKBP2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Testdurchführung
  1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
    2. Add recombinant FKBP2 protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
    3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
    4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
    5. Record the increase in A410 nm for 20 minutes
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Supplied as a liquid in 20 mM Tris buffer, pH 8.0, containing 10 % glycerol and 0 mM DTT.
Konservierungsmittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Avoid repeated freeze/thaw cycles.
Lagerung RT/-20 °C
Informationen zur Lagerung Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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SDS-PAGE (SDS) image for FK506 Binding Protein 2, 13kDa (FKBP2) (AA 22-142) Peptid (ABIN935096) Figure annotation denotes ug of protein loaded and % gel used.