anti-Huhn Stratifin Antikörper für Western Blotting

Recommended Stratifin Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Stratifin (SFN) Antikörper
  • YWHAS
  • Er
  • Mme1
  • Ywhas
  • stratifin
  • SFN
  • Sfn
Epitop
N-Term
23
21
19
17
14
14
13
6
4
4
3
3
3
3
3
3
1
1
1
1
Reaktivität
Huhn, Rind (Kuh), Human, Maus, Kaninchen, Ratte (Rattus), Schaf
198
67
52
14
14
13
10
10
8
7
4
4
4
2
2
2
1
1
1
1
1
Wirt
Kaninchen
146
37
21
2
1
Klonalität
Polyklonal
Konjugat
Dieser Stratifin Antikörper ist unkonjugiert
12
9
7
7
6
6
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
176
81
71
50
44
22
21
18
13
4
3
3
2
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN461974
501,70 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN318722 WB Rabbit IgG N-Term, acetylated Anmelden zum Anzeigen Polyclonal
1 ABIN1739741 IHC (p) WB Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2467428 IHC WB Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2477187 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 3

anti-Stratifin Antikörper für Western Blotting mit den meisten Publikationen

Ähnliche anti-Stratifin Antikörper

Applikation / Reaktivität Human Maus Ratte (Rattus) Rind (Kuh) Huhn Kaninchen Schaf
Immunofluorescence (fixed cells) (IF/ICC) 3 Antikörper 2 Antikörper 1 Antikörper
Western Blotting (WB) 169 Antikörper 15 Antikörper 5 Antikörper 51 Antikörper 11 Antikörper 38 Antikörper 14 Antikörper
Proximity Ligation Assay (PLA) 2 Antikörper
Immunoprecipitation (IP) 20 Antikörper 5 Antikörper 2 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antikörper 15 Antikörper 13 Antikörper 6 Antikörper 9 Antikörper 2 Antikörper

General

Antigen Stratifin (SFN) Antikörper
Epitop N-Term
(23), (21), (19), (17), (14), (14), (13), (6), (4), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1)
Reaktivität Huhn, Rind (Kuh), Human, Maus, Kaninchen, Ratte (Rattus), Schaf
(198), (67), (52), (14), (14), (13), (10), (10), (8), (7), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Wirt Kaninchen
(146), (37), (21), (2), (1)
Klonalität
Polyklonal
Konjugat Dieser Stratifin Antikörper ist unkonjugiert
(12), (9), (7), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(176), (81), (71), (50), (44), (22), (21), (18), (13), (4), (3), (3), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Stratifin Antikörper

Target Details Stratifin Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Recognizes 14-3-3 sigma in all mammals. It is a member of the 14-3-3 family which consists of 30 kD proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 sigma is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary. 14-3-3 sigma binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3 sigma inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3 sigma through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3 sigma targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1. May not react with recombinant proteins that are not N-acetylated.
Immunogen Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 sigma.

Type of Immunogen: Synthetic peptide
Isotyp IgG

Target Details Stratifin

Produktdetails anti-Stratifin Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung SFN / Stratifin / 14-3-3 Sigma (SFN Antibody Abstract)
Hintergrund Name/Gene ID: SFN

Synonyms: SFN, 14-3-3 sigma, 14-3-3 protein sigma, HME1, Stratifin, YWHAS
Gen-ID 2810
UniProt P31947
Pathways p53 Signalweg, Myometrial Relaxation and Contraction

Anwendungsinformationen

Produktdetails anti-Stratifin Antikörper Target Details Stratifin Handhabung Bilder zurück nach oben
Applikationshinweise Approved: IHC-P (1:250), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:250. Western Blot: LS-B3281 detects a band of approximately 30kD in HEK293 cell lysates.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Stratifin Antikörper Target Details Stratifin Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer Serum, 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeat freeze-thaw cycles.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Bilder

Produktdetails anti-Stratifin Antikörper Target Details Stratifin Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Stratifin Antikörper (SFN) (N-Term) (ABIN461974) Human Skin (formalin-fixed, paraffin-embedded) stained with SFN antibody ABIN461974 a...