anti-Ratte (Rattus) MDM2 Antikörper für Immunohistochemistry (Frozen Sections)

Recommended MDM2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
  • ACTFS
  • HDMX
  • hdm2
  • zgc:109834
  • 1700007J15Rik
  • AA415488
  • Mdm-2
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse)
  • p53 E3 ubiquitin protein ligase
  • transformed 3T3 cell double minute 2 homolog (mouse)
  • transformed mouse 3T3 cell double minute 2
  • MDM2
  • mdm2
  • Mdm2
Epitop
AA 154-167
46
42
36
29
26
24
15
15
14
12
8
8
7
7
6
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
379
135
94
14
8
6
4
2
2
2
2
2
2
1
1
1
Wirt
Maus
307
76
14
2
Klonalität
Monoklonal
Konjugat
Dieser MDM2 Antikörper ist unkonjugiert
12
12
12
9
9
9
3
3
3
3
3
3
3
3
3
3
3
2
2
Applikation
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
263
137
108
84
71
40
38
24
13
12
12
11
4
4
2
1
1
1
1
1
1
Hersteller
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Bilder

Produktnummer ABIN180331
$ 456.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN967511 BI IHC (f) IHC (fro) IP WB Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14 2
1 ABIN214211 IHC (fro) IHC (rs) IHC (p) IP Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14

General

Antigen Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
Epitop AA 154-167
(46), (42), (36), (29), (26), (24), (15), (15), (14), (12), (8), (8), (7), (7), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(379), (135), (94), (14), (8), (6), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Wirt Maus
(307), (76), (14), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser MDM2 Antikörper ist unkonjugiert
(12), (12), (12), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Applikation Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(263), (137), (108), (84), (71), (40), (38), (24), (13), (12), (12), (11), (4), (4), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MDM2 Antikörper

Target Details MDM2 Anwendungsinformationen Handhabung Referenzen für anti-MDM2 Antikörper (ABIN180331) Bilder
Spezifität This antibody recognizes the 90 kDa MDM2 protein, which forms a complex with p53 leading to p53 inhibition. May also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer.
Cellular Localization: nuclear.
Produktmerkmale Synonyms: p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, Hdm2, E3ubiquitin-protein ligase Mdm2
Reinigung Affinity Chromatography on Protein G.
Immunogen A Synthetic peptide corresponding to amino acids 154-167 of human MDM2 protein. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8. 653myeloma cell line. AA Sequence: Cys-SRPSTSSRRRAISE
Klon SMP14
Isotyp IgG1

Target Details MDM2

Produktdetails anti-MDM2 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-MDM2 Antikörper (ABIN180331) Bilder zurück nach oben
Antigen
Andere Bezeichnung MDM2 (MDM2 Antibody Abstract)
Hintergrund Human homolog of Murine Double Minute-2 (MDM2) and p53 function in mutual regulation: activation of p53 results in expression of MDM2 which consequently inhibits the ability of wild type p53 to stimulate expression of target genes. MDM2 is frequently overexpressed in the nuclei of soft tissue sarcomas, and to a much lesser extent, in a wide variety of malignancies of bladder, breast, testes, cervix, and glial cells.Synonyms: Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2
Gen-ID 4193
UniProt Q00987
Forschungsgebiet Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie

Anwendungsinformationen

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Handhabung Referenzen für anti-MDM2 Antikörper (ABIN180331) Bilder zurück nach oben
Applikationshinweise Western Blot: 1/1000. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200-1/500. This product requires antigenretrieval using heat treatment prior to staining of paraffin sections. EDTA pH 8.0 isrecommended for this purpose. Recommended Positive Control: Breast Carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Referenzen für anti-MDM2 Antikörper (ABIN180331) Bilder zurück nach oben
Konzentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Haltbarkeit 12 months

Referenzen für anti-MDM2 Antikörper (ABIN180331)

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Gueven, Becherel, Birrell, Chen, DelSal, Carney, Grattan-Smith, Lavin: "Defective p53 response and apoptosis associated with an ataxia-telangiectasia-like phenotype." in: Cancer research, Vol. 66, Issue 6, pp. 2907-12, 2006

Martin, Trouche, Hagemeier, Sørensen, La Thangue, Kouzarides: "Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein." in: Nature, Vol. 375, Issue 6533, pp. 691-4, 1995

Picksley, Vojtesek, Sparks, Lane: "Immunochemical analysis of the interaction of p53 with MDM2;--fine mapping of the MDM2 binding site on p53 using synthetic peptides." in: Oncogene, Vol. 9, Issue 9, pp. 2523-9, 1994

Bilder

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Handhabung Referenzen für anti-MDM2 Antikörper (ABIN180331) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 154-167) antibody (ABIN180331) anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 154-167) antibody