anti-Maus MDM2 Antikörper für Immunoprecipitation

Recommended MDM2 Antibody (geliefert von: Anmelden zum Anzeigen )

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Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
  • ACTFS
  • HDMX
  • hdm2
  • zgc:109834
  • 1700007J15Rik
  • AA415488
  • Mdm-2
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse)
  • p53 E3 ubiquitin protein ligase
  • transformed 3T3 cell double minute 2 homolog (mouse)
  • transformed mouse 3T3 cell double minute 2
  • MDM2
  • mdm2
  • Mdm2
Epitop
Center
42
37
36
27
25
24
19
15
15
15
8
8
7
7
6
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
370
133
94
14
8
6
4
2
2
2
2
2
2
1
1
1
Wirt
Kaninchen
298
76
14
2
Klonalität
Polyklonal
Konjugat
Dieser MDM2 Antikörper ist unkonjugiert
12
12
12
9
9
9
3
3
3
3
3
3
3
3
3
3
3
2
2
Applikation
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
256
133
103
76
70
40
38
22
12
12
11
10
4
4
2
1
1
1
1
1
Optionen
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Bilder

Produktnummer ABIN2854782
357,24 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN180331 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14 3
1 ABIN967511 BI IHC (f) IHC (fro) IP WB Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14 2
1 ABIN2475548 IHC (fro) IHC (rs) IHC (p) IP Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14 3
1 ABIN1724157 IHC (fro) IHC (p) IP IHC WB Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14
1 ABIN2312856 IP ELISA WB Rabbit IgG AA 177-195 Anmelden zum Anzeigen Polyclonal

anti-MDM2 Antikörper für Immunoprecipitation mit den meisten Publikationen

Ähnliche anti-MDM2 Antikörper

Applikation / Reaktivität Maus Human
BioImaging (BI) 1 Antikörper 1 Antikörper
Chromatin Immunoprecipitation (ChIP) 1 Antikörper 1 Antikörper
ELISA 35 Antikörper
Enzyme Immunoassay (EIA) 3 Antikörper
Immunocytochemistry (ICC) 4 Antikörper
Immunofluorescence (IF) 26 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 7 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antikörper
Immunohistochemistry (IHC) 30 Antikörper
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antikörper
Immunohistochemistry (Resin Sections) (IHC (rs)) 1 Antikörper
Immunoprecipitation (IP) 6 Antikörper
Western Blotting (WB) 92 Antikörper
Dot Blot (DB) 38 Antikörper

General

Antigen Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
Epitop Center
(42), (37), (36), (27), (25), (24), (19), (15), (15), (15), (8), (8), (7), (7), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(370), (133), (94), (14), (8), (6), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Wirt Kaninchen
(298), (76), (14), (2)
Klonalität
Polyklonal
Konjugat Dieser MDM2 Antikörper ist unkonjugiert
(12), (12), (12), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Applikation Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(256), (133), (103), (76), (70), (40), (38), (22), (12), (12), (11), (10), (4), (4), (2), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MDM2 Antikörper

Target Details MDM2 Anwendungsinformationen Handhabung Bilder
Kreuzreaktivität Katze, Hund, Schwein
Kreuzreaktivität (Details) Cat (95 %), Dog (91 %), Pig (93 %)
Produktmerkmale Rabbit Polyclonal antibody to MDM2 (Mdm2 p53 binding protein homolog)
MDM2 antibody
Reinigung Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.
Isotyp IgG

Target Details MDM2

Produktdetails anti-MDM2 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
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Andere Bezeichnung MDM2 (MDM2 Antibody Abstract)
Hintergrund This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

Cellular Localization: Nucleus , nucleoplasm , Cytoplasm , nucleolus
Molekulargewicht 55 kDa
Gen-ID 4193
Forschungsgebiet Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie

Anwendungsinformationen

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Handhabung Bilder zurück nach oben
Applikationshinweise Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:1000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:1000*
Kommentare

Positive Control: H1299 , HCT116 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , mouse MDM2-transfected 293T

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 0.74 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-MDM2 Antikörper (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse)) (Center) (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg 293T w...
Immunohistochemistry (IHC) image for anti-MDM2 Antikörper (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse)) (Center) (ABIN2854782) IHC-P Image Immunohistochemical analysis of paraffin-embedded human lung cancer, usin...
Immunofluorescence (IF) image for anti-MDM2 Antikörper (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse)) (Center) (ABIN2854782) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MDM2, ...
Western Blotting (WB) image for anti-MDM2 Antikörper (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse)) (Center) (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg Neuro2...
Western Blotting (WB) image for anti-MDM2 Antikörper (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse)) (Center) (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg H1299 ...
Chromatin Immunoprecipitation (ChIP) image for anti-MDM2 Antikörper (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse)) (Center) (ABIN2854782) ChIP Image Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of ei...
Immunoprecipitation (IP) image for anti-MDM2 Antikörper (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse)) (Center) (ABIN2854782) IP Image MDM2 antibody immunoprecipitates MDM2 protein in IP experiments. IP samples:...