anti-Human MDM2 Antikörper für Flow Cytometry

Recommended MDM2 Antibody (geliefert von: Anmelden zum Anzeigen )

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Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
  • ACTFS
  • HDMX
  • hdm2
  • zgc:109834
  • 1700007J15Rik
  • AA415488
  • Mdm-2
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse)
  • p53 E3 ubiquitin protein ligase
  • transformed 3T3 cell double minute 2 homolog (mouse)
  • transformed mouse 3T3 cell double minute 2
  • MDM2
  • mdm2
  • Mdm2
Epitop
AA 141-176
46
42
36
29
26
24
15
15
15
12
8
8
7
7
6
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
379
136
95
14
8
6
4
2
2
2
2
2
2
1
1
1
Wirt
Kaninchen
306
77
14
2
Klonalität
Polyklonal
Konjugat
Dieser MDM2 Antikörper ist unkonjugiert
12
12
12
9
9
9
3
3
3
3
3
3
3
3
3
3
3
2
2
Applikation
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
263
137
108
83
71
40
38
25
13
12
12
12
4
4
2
1
1
1
1
1
Hersteller
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Bilder

Produktnummer ABIN5538374
$ 515.63
Zzgl. Versandkosten $45.00

General

Antigen Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
Epitop AA 141-176
(46), (42), (36), (29), (26), (24), (15), (15), (15), (12), (8), (8), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(379), (136), (95), (14), (8), (6), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Wirt Kaninchen
(306), (77), (14), (2)
Klonalität
Polyklonal
Konjugat Dieser MDM2 Antikörper ist unkonjugiert
(12), (12), (12), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Applikation Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(263), (137), (108), (83), (71), (40), (38), (25), (13), (12), (12), (12), (4), (4), (2), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MDM2 Antikörper

Target Details MDM2 Anwendungsinformationen Handhabung Bilder
Reinigung This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This MDM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-176 amino acids from human MDM2.
Isotyp Ig Fraction

Target Details MDM2

Produktdetails anti-MDM2 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung MDM2 (MDM2 Antibody Abstract)
Hintergrund MDM2 is a target of the transcription factor tumor protein p53. This protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
Molekulargewicht 55 kDa
Gen-ID 4193
UniProt Q00987
Forschungsgebiet Cell Cycle, Transcription Factors, Cancer
Pathways p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie

Anwendungsinformationen

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Handhabung Bilder zurück nach oben
Applikationshinweise For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 0.5 mg/mL
Buffer Supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Bilder

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
 image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 141-176) antibody (ABIN5538374) Overlay histogram showing THP-1 cells stained with Antibody (green line). The cells w...
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 141-176) antibody (ABIN5538374) Western Blot at 1:2000 dilution + CCRF-CEM whole cell lysate Lysates/proteins at 20 u...
 image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 141-176) antibody (ABIN5538374) Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the M...