anti-Human MDM2 Antikörper für Enzyme Immunoassay

Recommended MDM2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
  • ACTFS
  • HDMX
  • hdm2
  • zgc:109834
  • 1700007J15Rik
  • AA415488
  • Mdm-2
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse)
  • p53 E3 ubiquitin protein ligase
  • transformed 3T3 cell double minute 2 homolog (mouse)
  • transformed mouse 3T3 cell double minute 2
  • MDM2
  • mdm2
  • Mdm2
Epitop
pThr218
42
37
35
27
25
24
19
15
15
15
8
8
7
7
6
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
370
134
94
14
8
6
4
2
2
2
2
2
2
1
1
1
Wirt
Kaninchen
298
76
14
2
Klonalität
Polyklonal
Konjugat
Dieser MDM2 Antikörper ist unkonjugiert
12
12
12
9
9
9
3
3
3
3
3
3
3
3
3
3
3
2
2
Applikation
Enzyme Immunoassay (EIA)
257
133
103
77
71
40
38
23
12
12
11
10
4
4
2
1
1
1
1
1
1
Hersteller
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Bilder

Produktnummer ABIN358421
407,00 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN358505 DB EIA Rabbit Ig pSer395 Anmelden zum Anzeigen Polyclonal 3
1 ABIN356776 EIA IHC (p) Rabbit Ig Anmelden zum Anzeigen Polyclonal 5
1 ABIN781912 EIA IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 1A7
1 ABIN2725675 EIA IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN2725678 EIA Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN2725676 EIA Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN2725679 EIA Rabbit IgG Anmelden zum Anzeigen Polyclonal

anti-MDM2 Antikörper für Enzyme Immunoassay mit den meisten Publikationen

Ähnliche anti-MDM2 Antikörper

Applikation / Reaktivität Human
BioImaging (BI) 1 Antikörper
Chromatin Immunoprecipitation (ChIP) 1 Antikörper
Dot Blot (DB) 38 Antikörper
ELISA 118 Antikörper
Enzyme Immunoassay (EIA) 8 Antikörper
Flow Cytometry (FACS) 1 Antikörper
Immunochromatography (IC) 4 Antikörper
Immunocytochemistry (ICC) 11 Antikörper
Immunofluorescence (IF) 76 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 12 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antikörper
Immunohistochemistry (IHC) 103 Antikörper
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 12 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 70 Antikörper
Immunohistochemistry (Resin Sections) (IHC (rs)) 4 Antikörper

General

Antigen Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
Epitop pThr218
(42), (37), (35), (27), (25), (24), (19), (15), (15), (15), (8), (8), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(370), (134), (94), (14), (8), (6), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Wirt Kaninchen
(298), (76), (14), (2)
Klonalität
Polyklonal
Konjugat Dieser MDM2 Antikörper ist unkonjugiert
(12), (12), (12), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Applikation Enzyme Immunoassay (EIA)
(257), (133), (103), (77), (71), (40), (38), (23), (12), (12), (11), (10), (4), (4), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MDM2 Antikörper

Target Details MDM2 Anwendungsinformationen Handhabung ProductDetails: References for anti-MDM2 antibody (ABIN358421) Bilder
Spezifität This antibody detects MDM2 pThr218.
Reinigung Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T218 of human MDM2.
Isotyp Ig

Target Details MDM2

Produktdetails anti-MDM2 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-MDM2 antibody (ABIN358421) Bilder zurück nach oben
Antigen
Andere Bezeichnung MDM2 (MDM2 Antibody Abstract)
Hintergrund MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.Synonyms: Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2
Molekulargewicht 55232 Da
Gen-ID 4193, 9606
UniProt Q00987
Forschungsgebiet Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie

Anwendungsinformationen

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Handhabung ProductDetails: References for anti-MDM2 antibody (ABIN358421) Bilder zurück nach oben
Applikationshinweise ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen ProductDetails: References for anti-MDM2 antibody (ABIN358421) Bilder zurück nach oben
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-MDM2 antibody (ABIN358421)

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Burch, Scott, Pohler, Meek, Hupp: "Phage-peptide display identifies the interferon-responsive, death-activated protein kinase family as a novel modifier of MDM2 and p21WAF1." in: Journal of molecular biology, Vol. 337, Issue 1, pp. 115-28, 2004

Schon, Friedler, Freund, Fersht: "Binding of p53-derived ligands to MDM2 induces a variety of long range conformational changes." in: Journal of molecular biology, Vol. 336, Issue 1, pp. 197-202, 2004

Mantesso, Loducca, Bendit, Garicochea, Nunes, de Araújo: "Mdm2 mRNA expression in salivary gland tumour cell lines." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 33, Issue 2, pp. 96-101, 2004

Shmueli, Oren: "Regulation of p53 by Mdm2: fate is in the numbers." in: Molecular cell, Vol. 13, Issue 1, pp. 4-5, 2004

Xia, Paik, Li: "p53 activation in chronic radiation-treated breast cancer cells: regulation of MDM2/p14ARF." in: Cancer research, Vol. 64, Issue 1, pp. 221-8, 2004

Bilder

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Handhabung ProductDetails: References for anti-MDM2 antibody (ABIN358421) zurück nach oben
Bilder des Herstellers
 image for anti-MDM2 Antikörper (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse)) (pThr218) (ABIN358421) anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pThr218) antibody