anti-Human Tumor Necrosis Factor Antikörper für Inhibition Assay

Recommended Tumor Necrosis Factor Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tumor Necrosis Factor (TNF) Antikörper
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reaktivität
Human
1258
465
359
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Wirt
Maus
941
521
186
49
10
9
7
6
4
3
2
Klonalität
Monoklonal
Konjugat
Dieser Tumor Necrosis Factor Antikörper ist unkonjugiert
169
100
78
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
8
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Flow Cytometry (FACS), Immunofluorescence (IF), Inhibition Assay (InhA), Neutralization (Neut)
888
860
632
323
287
230
220
191
138
118
97
69
30
29
26
22
20
18
14
9
8
7
6
6
6
5
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN3026400
$ 361.90
Zzgl. Versandkosten $45.00
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General

Antigen Tumor Necrosis Factor (TNF) Antikörper
Reaktivität Human
(1258), (465), (359), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Wirt Maus
(941), (521), (186), (49), (10), (9), (7), (6), (4), (3), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Tumor Necrosis Factor Antikörper ist unkonjugiert
(169), (100), (78), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (8), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Applikation Flow Cytometry (FACS), Immunofluorescence (IF), Inhibition Assay (InhA), Neutralization (Neut)
(888), (860), (632), (323), (287), (230), (220), (191), (138), (118), (97), (69), (30), (29), (26), (22), (20), (18), (14), (9), (8), (7), (6), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Tumor Necrosis Factor Antikörper

Target Details Tumor Necrosis Factor Anwendungsinformationen Handhabung Bilder
Keine Kreuzreaktivität Maus
Produktmerkmale This antibody neutralises human TNFa mediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of hTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Reinigung Protein G affinity chromatography
Immunogen Recombinant human protein was used as the immunogen for the TNF-alpha antibody.
Klon J2D10
Isotyp IgG1, kappa

Target Details Tumor Necrosis Factor

Produktdetails anti-Tumor Necrosis Factor Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung TNF-alpha (TNF Antibody Abstract)
Hintergrund This antibody neutralises human TNFa mediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of hTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Forschungsgebiet Inflammation, Cancer, Virology
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Anwendungsinformationen

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Handhabung Bilder zurück nach oben
Applikationshinweise Optimal dilution of the TNF-alpha antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Functional Activity: Inhibits Tumor Growth, Neutralizes rTNF-alpha Mediated Cytotoxicity, Protects Mice Against Toxicity of rhTNF-alpha (Order SAF formulation)
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Anwendungsinformationen Bilder zurück nach oben
Konzentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store the TNF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).