Maus RANK ELISA Kit

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Epitop
AA 30-213
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
9
3
2
Methodentyp
Sandwich ELISA
Detektionsbereich
62.5-4000 pg/mL
Untere Nachweisgrenze
62.5 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Produktnummer ABIN921106
529,05 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
9.702543 ABIN2114827 Cell Culture Supernatant, Serum Anmelden zum Anzeigen
1 ABIN2042648 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN2116323 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen

RANK ELISA Kit für ELISA mit den meisten Publikationen

  • Mouse (Murine) RANK ELISA Kit für Sandwich ELISA - ABIN921106 (1 Publications): Darnay, Haridas, Ni, Moore, Aggarwal et al.: Characterization of the intracellular domain of receptor activator of NF-kappaB (RANK). Interaction with tumor necrosis factor receptor-associated factors and activation of NF-kappab and c-Jun ... in The Journal of biological chemistry 1998 (PubMed)

Ähnliche RANK ELISA Kits

Applikation / Reaktivität Human Ratte (Rattus)
ELISA 9 ELISA Kits 2 ELISA Kits

General

Antigen RANK (CD265) ELISA Kits
Epitop AA 30-213
Reaktivität Maus
Kits mit alternativen Reaktivitäten:
(9), (3), (2)
Methodentyp Sandwich ELISA
Detektionsbereich 62.5-4000 pg/mL
Untere Nachweisgrenze 62.5 pg/mL
Applikation ELISA
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails RANK ELISA Kit

Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for RANK Kit (ABIN921106) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse RANK
Marke PicoKine™
Proben Cell Culture Supernatant, Serum
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: NSO
Immunogen sequence: Q30-P213
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <2pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: Q30-P213

Antigendetails

Produktdetails RANK ELISA Kit Anwendungsinformationen Handhabung ProductDetails: References for RANK Kit (ABIN921106) Bilder zurück nach oben
Antigen
Andere Bezeichnung RANK (CD265 ELISA Kit Abstract)
Hintergrund

Protein Function: Receptor for TNFSF11/RANKL/TRANCE/OPGL, essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. .

Background: Receptor Activator of Nuclear Factor kappa B(RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK.

Synonyms: Tumor necrosis factor receptor superfamily member 11A,Osteoclast differentiation factor receptor,ODFR,Receptor activator of NF-KB,CD265,Tnfrsf11a,Rank,

Full Gene Name: Tumor necrosis factor receptor superfamily member 11A

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.
Gen-ID 21934
UniProt O35305
Pathways NF-kappaB Signalweg

Anwendungsinformationen

Produktdetails RANK ELISA Kit Antigendetails Handhabung ProductDetails: References for RANK Kit (ABIN921106) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Tissue Specificity: Ubiquitous expression with high levels in trabecular bone, thymus, small intestine, lung, brain and kidney. Weakly expressed in spleen and bone marrow.

Plattentyp Pre-coated
Protokoll mouse RANK ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for RANK has been precoated onto 96-well plates. Standards(NSO, Q30-P213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RANK is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse RANK amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse RANK standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse RANK standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 735, Standard deviation: 38.22, CV(%): 5.2
  • Sample 2: n=16, Mean(pg/ml): 1428, Standard deviation: 87.11, CV(%): 6.1
  • Sample 3: n=16, Mean(pg/ml): 2873, Standard deviation: 140.8, CV(%): 4.9,
  • Sample 1: n=24, Mean(pg/ml): 873, Standard deviation: 57.62, CV(%): 6.6
  • Sample 2: n=24, Mean(pg/ml): 1740, Standard deviation: 125.28, CV(%): 7.2
  • Sample 3: n=24, Mean(pg/ml): 3226, Standard deviation: 170.98, CV(%): 5.3
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails RANK ELISA Kit Antigendetails Anwendungsinformationen ProductDetails: References for RANK Kit (ABIN921106) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

ProductDetails: References for RANK Kit (ABIN921106)

Produktdetails RANK ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Darnay, Haridas, Ni, Moore, Aggarwal et al.: "Characterization of the intracellular domain of receptor activator of NF-kappaB (RANK). Interaction with tumor necrosis factor receptor-associated factors and activation of NF-kappab and c-Jun ..." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20551-5, 1998

Bilder

Produktdetails RANK ELISA Kit Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for RANK Kit (ABIN921106) zurück nach oben
Bilder des Herstellers
ELISA image for RANK ELISA Kit (CD265) (ABIN921106) Mouse RANK PicoKine ELISA Kit standard curve