Ratte (Rattus) p65 ELISA Kit

Recommended p65 Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) ELISA Kits
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Reaktivität
Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
57
43
35
4
3
3
3
2
2
2
2
Methodentyp
Sandwich ELISA
Detektionsbereich
1.56-100 pg/mL
Untere Nachweisgrenze
1.56 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN368280
$ 1,107.33
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.090268 ABIN416166 Sandwich ELISA Tissue Homogenate, Cell Lysate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Anmelden zum Anzeigen
10.090268 ABIN1572169 Sandwich ELISA Biological Fluids, Cell Lysate, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN627317 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Anmelden zum Anzeigen
1 ABIN772132 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 ng/mL 50 ng/mL Anmelden zum Anzeigen
1 ABIN1116224 Sandwich ELISA Serum, Plasma, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Anmelden zum Anzeigen
1 ABIN2748430 Sandwich ELISA Cell Lysate, Tissue Lysate Anmelden zum Anzeigen
1 ABIN2748431 Sandwich ELISA Cell Lysate, Tissue Lysate Anmelden zum Anzeigen
1 ABIN1380658 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380659 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380656 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380657 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380147 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380660 Cell ELISA Anmelden zum Anzeigen
1 ABIN2748433 Sandwich ELISA Cell Lysate, Tissue Lysate Anmelden zum Anzeigen
1 ABIN5526742 Cell ELISA Adherent Cell Culture Anmelden zum Anzeigen
1 ABIN5524698 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 78.125-5000 pg/mL 78.125 pg/mL Anmelden zum Anzeigen
1 ABIN5659816 Sandwich ELISA Biological Fluids, Cell Lysate, Tissue Homogenate 0.156 ng/mL - 10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN2114653 Cell ELISA Anmelden zum Anzeigen
1 ABIN2114650 Cell ELISA Anmelden zum Anzeigen
1 ABIN2114647 Cell ELISA Anmelden zum Anzeigen

General

Antigen Nuclear Factor-KB P65 (NFkBP65) ELISA Kits
Reaktivität Ratte (Rattus)
Kits mit alternativen Reaktivitäten:
(57), (43), (35), (4), (3), (3), (3), (2), (2), (2), (2)
Methodentyp Sandwich ELISA
Detektionsbereich 1.56-100 pg/mL
Untere Nachweisgrenze 1.56 pg/mL
Applikation ELISA
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails p65 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Referenzen für p65 Kit (ABIN368280) Bilder
Verwendungszweck For the quantitative determination of rat nuclear factor-kappaB p65 (NF-kappaB p65) concentrations in serum, plasma, tissue homogenates.
Proben Serum, Plasma, Tissue Homogenate
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This assay has high sensitivity and excellent specificity for detection of rat NF-kappaB p65.
Kreuzreaktivität (Details) Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
Sensitivität 0.39 pg/mL
Bestandteile
  • Assay plate (12 × 8 coated Microwells)
  • Standard (freeze dried)
  • Biotin-antibody (100 × concentrate)
  • HRP-avidin (100 × concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin Diluent
  • Sample Diluent
  • Wash Buffer (25 × concentrate)
  • TMB Substrate
  • Stop Solution
  • Adhesive Strip (for 96 wells)
  • Instruction manual
Benötigtes Material
  • Microplate reader capable of measuring absorbance at 450nm, with the correction wavelength set at 540nm or 570nm.
  • An incubator which can provide stable incubation conditions up to 37°C ± 0.5°C.
  • Squirt bottle, manifold dispenser or automated microplate washer.
  • Absorbent paper for blotting the microtiter plate.
  • 100mL and 500mL graduated cylinders.
  • Deionized or distilled water.
  • Pipettes and pipette tips.
  • Test tubes for dilution.

Antigendetails

Produktdetails p65 ELISA Kit Anwendungsinformationen Handhabung Referenzen für p65 Kit (ABIN368280) Bilder zurück nach oben
Antigen
Andere Bezeichnung Nuclear Factor-KappaB p65 (NF-KappaB p65) (NFkBP65 ELISA Kit Abstract)
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails p65 ELISA Kit Antigendetails Handhabung Referenzen für p65 Kit (ABIN368280) Bilder zurück nach oben
Applikationshinweise
  • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
  • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
  • Grossly hemolyzed samples are not suitable for use in this assay.
  • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
  • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
  • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
  • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
  • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
  • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
Kommentare

Detection wavelength: 450 nm

Information on standard material:
Some standards are from natural resource, some are recombinant protein, but the recombinant protein will not expressed from Baculovirus. Generally it's CHO cells, but that depends.The formulation of auxiliary material in the standard is classified, but it dosen't contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

Information on reagents:
Most of the stop solution are 1 N H2SO4, a few is not. The formulation of wash solution is classified. None of the components contains (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. Some could contain BSA. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

Information on antibodies:
The provided antibodies and their host vary in different kits. Some are affinity purified, some are Protein A or G purified

Probenmenge 100 μL
Testdauer 1 - 4.5 h
Plattentyp Pre-coated
Protokoll This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-kappaB p65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-kappaB p65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-kappaB p65 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-kappaB p65 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Probennahme
  • Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
  • Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
  • Tissue Homogenates: Rinse 100 mg tissue with 1× PBS, homogenize in 1mL of 1× PBS and store overnight at -20 °C. After two freeze-thaw cycles to break the cell membranes, centrifuge the homogenates for 5 minutes at 5000 × g, 2-8 °C. Remove and assay the supernate immediately. Alternatively, aliquot and store samples at -20 °C or -80 °C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
Ergebnisberechnung Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Testpräzision Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
  • Intra-assay: CV% less than 8%
  • Inter-assay: CV% less than 10%
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Handhabung

Produktdetails p65 ELISA Kit Antigendetails Anwendungsinformationen Referenzen für p65 Kit (ABIN368280) Bilder zurück nach oben
Vorsichtsmaßnahmen The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Handhabung
  • The kit should not be used beyond the expiration date on the kit label.
  • Do not mix or substitute reagents with those from other lots or sources.
  • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
  • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
  • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.
Haltbarkeit 6 months

Referenzen für p65 Kit (ABIN368280)

Produktdetails p65 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Ahmed, El Morsy, Ahmed: "Pomegranate extract protects against cerebral ischemia/reperfusion injury and preserves brain DNA integrity in rats." in: Life sciences, Vol. 110, Issue 2, pp. 61-9, 2014

Bilder

Produktdetails p65 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für p65 Kit (ABIN368280) zurück nach oben
Bilder des Herstellers
ELISA image for Nuclear Factor-KB P65 (NFkBP65) ELISA Kit (ABIN368280) Typical standard curve