Maus p65 ELISA Kit

Recommended p65 Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) ELISA Kits
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
57
38
32
4
3
3
3
3
2
2
2
Methodentyp
Sandwich ELISA
Detektionsbereich
0.156-10 ng/mL
Untere Nachweisgrenze
0.156 ng/mL
Applikation
ELISA
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1572168
822,13 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
9.702586 ABIN367915 Sandwich ELISA Serum, Plasma, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN628131 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Anmelden zum Anzeigen
1 ABIN1116222 Sandwich ELISA Serum, Plasma, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Anmelden zum Anzeigen
1 ABIN773475 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 1.0-25 ng/mL 1.0 ng/mL Anmelden zum Anzeigen
1 ABIN2748430 Sandwich ELISA Cell Lysate, Tissue Lysate Anmelden zum Anzeigen
1 ABIN2748431 Sandwich ELISA Cell Lysate, Tissue Lysate Anmelden zum Anzeigen
1 ABIN1380660 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380650 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380656 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380657 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380658 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380659 Cell ELISA Anmelden zum Anzeigen
1 ABIN1380147 Cell ELISA Anmelden zum Anzeigen
1 ABIN2748433 Sandwich ELISA Cell Lysate, Tissue Lysate Anmelden zum Anzeigen
1 ABIN1381357 Cell ELISA Anmelden zum Anzeigen
1 ABIN5526742 Cell ELISA Adherent Cell Culture Anmelden zum Anzeigen
1 ABIN5524697 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.313-20 ng/mL 0.313 ng/mL Anmelden zum Anzeigen
1 ABIN2114648 Cell ELISA Anmelden zum Anzeigen
1 ABIN2114649 Cell ELISA Anmelden zum Anzeigen
1 ABIN2114650 Cell ELISA Anmelden zum Anzeigen

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General

Antigen Nuclear Factor-KB P65 (NFkBP65) ELISA Kits
Reaktivität Maus
Kits mit alternativen Reaktivitäten:
(57), (38), (32), (4), (3), (3), (3), (3), (2), (2), (2)
Methodentyp Sandwich ELISA
Detektionsbereich 0.156-10 ng/mL
Untere Nachweisgrenze 0.156 ng/mL
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails p65 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of RELA in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Proben Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This assay has high sensitivity and excellent specificity for detection of this index.
Kreuzreaktivität (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivität 0.041 ng/mL
Bestandteile
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Benötigtes Material
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Antigendetails

Produktdetails p65 ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung RELA (NFkBP65 ELISA Kit Abstract)
Hintergrund Synonyms: NFKB3, p65, REL-A, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3,
Gen-ID 19697
UniProt Q548Y4
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails p65 ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Probenmenge 100 μL
Testdauer 1 - 4.5 h
Plattentyp Pre-coated
Protokoll 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Testdurchführung The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Testpräzision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails p65 ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Vorsichtsmaßnahmen The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handhabung The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Haltbarkeit 12 months

Bilder

Produktdetails p65 ELISA Kit Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
ELISA image for p65 ELISA Kit (Nuclear Factor-KB P65) (ABIN1572168) Nuclear Factor-KB P65 (NFkBP65) ELISA Kit