anti-Kaninchen p65 Antikörper für BioImaging

Recommended p65 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antikörper
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Epitop
AA 136-224
137
72
66
58
51
47
43
40
39
36
33
32
25
21
18
17
16
15
15
13
13
13
12
11
10
9
9
8
7
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Hund, Frosch, Human, Kaninchen, Ratte (Rattus)
1207
677
518
73
59
55
30
30
28
24
15
8
8
6
5
4
1
1
1
Wirt
Maus
1037
146
17
16
11
1
Klonalität
Monoklonal
Konjugat
Dieser p65 Antikörper ist unkonjugiert
34
34
26
21
19
18
16
16
13
13
13
9
9
9
9
9
9
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Applikation
BioImaging (BI), Western Blotting (WB)
880
429
357
269
224
149
118
88
84
28
22
19
18
11
11
6
6
6
5
4
4
2
1
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN968222
146,73 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN968223 BI WB Mouse IgG1 AA 136-224 Anmelden zum Anzeigen 20-NF 5

anti-p65 Antikörper für BioImaging mit den meisten Publikationen

Ähnliche anti-p65 Antikörper

Applikation / Reaktivität Human Ratte (Rattus) Hund Kaninchen Frosch
Immunofluorescence (fixed cells) (IF/ICC) 17 Antikörper 10 Antikörper
Cytometry by Time of Flight (CyTOF) 4 Antikörper 4 Antikörper
Simple Western (SimWes) 6 Antikörper 4 Antikörper
Western Blotting (WB) 862 Antikörper 367 Antikörper 27 Antikörper 23 Antikörper 2 Antikörper
Proximity Ligation Assay (PLA) 5 Antikörper 2 Antikörper 2 Antikörper
Microarray (MA) 1 Antikörper
Intracellular Staining (ICS) 4 Antikörper
Immunoprecipitation (IP) 148 Antikörper

General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antikörper
Epitop AA 136-224
(137), (72), (66), (58), (51), (47), (43), (40), (39), (36), (33), (32), (25), (21), (18), (17), (16), (15), (15), (13), (13), (13), (12), (11), (10), (9), (9), (8), (7), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Hund, Frosch, Human, Kaninchen, Ratte (Rattus)
(1207), (677), (518), (73), (59), (55), (30), (30), (28), (24), (15), (8), (8), (6), (5), (4), (1), (1), (1)
Wirt Maus
(1037), (146), (17), (16), (11), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser p65 Antikörper ist unkonjugiert
(34), (34), (26), (21), (19), (18), (16), (16), (13), (13), (13), (9), (9), (9), (9), (9), (9), (8), (8), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Applikation BioImaging (BI), Western Blotting (WB)
(880), (429), (357), (269), (224), (149), (118), (88), (84), (28), (22), (19), (18), (11), (11), (6), (6), (6), (5), (4), (4), (2), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p65 Antikörper

Target Details p65 Anwendungsinformationen Handhabung ProductDetails: References for anti-p65 antibody (ABIN968222) Bilder
Kreuzreaktivität Hund, Frosch, Kaninchen, Ratte (Rattus)
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human NF-kappaB aa. 136-224
Klon 20-NF
Isotyp IgG1

Target Details p65

Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-p65 antibody (ABIN968222) Bilder zurück nach oben
Antigen
Andere Bezeichnung NF-kappa B p65 (NFkBP65 Antibody Abstract)
Hintergrund NF-kappaB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. The most studied NF-kappaB complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for the interaction of NF-kappaB with its inhibitor, IkappaB. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkappaB. This complex prevents nuclear translocation and activity of NF-kappaB. In response to stimuli such as cytokines, LPS, and viral infections, IkappaB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkappaB/NF-kappaB complex, allowing the free heterodimeric NF-kappaB to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the kappaB site within promoters and enhancers and functions as a transcriptional activator.
Molekulargewicht 65 kDa
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-p65 Antikörper Target Details p65 Handhabung ProductDetails: References for anti-p65 antibody (ABIN968222) Bilder zurück nach oben
Applikationshinweise Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Kommentare

Related Products: ABIN968537, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen ProductDetails: References for anti-p65 antibody (ABIN968222) Bilder zurück nach oben
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20°C.

ProductDetails: References for anti-p65 antibody (ABIN968222)

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Nishibe, Parry, Ishida, Aziz, Murray, Patel, Rahman, Strand, Saito, Saito, Hammond, Savidge, Mackman, Wijelath: "Oncostatin M promotes biphasic tissue factor expression in smooth muscle cells: evidence for Erk-1/2 activation." in: Blood, Vol. 97, Issue 3, pp. 692-9, 2001

Pimentel-Muiños, Seed: "Regulated commitment of TNF receptor signaling: a molecular switch for death or activation." in: Immunity, Vol. 11, Issue 6, pp. 783-93, 2000

Kieran, Blank, Logeat, Vandekerckhove, Lottspeich, Le Bail, Urban, Kourilsky, Baeuerle, Israël: "The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product." in: Cell, Vol. 62, Issue 5, pp. 1007-18, 1990

Shirakawa, Mizel: "In vitro activation and nuclear translocation of NF-kappa B catalyzed by cyclic AMP-dependent protein kinase and protein kinase C." in: Molecular and cellular biology, Vol. 9, Issue 6, pp. 2424-30, 1989

Baeuerle, Baltimore: "Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor." in: Cell, Vol. 53, Issue 2, pp. 211-7, 1988

Bilder

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung ProductDetails: References for anti-p65 antibody (ABIN968222) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (AA 136-224) (ABIN968222) Western blot analysis of NF-kappaB p65 on a Jurkat lysate. Lane 1: 1:250, lane 2: 1:5...
Immunofluorescence (IF) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (AA 136-224) (ABIN968222) Immunofluorescent staining of A549 (ATCC CCL-185) cells. Cells were seeded in a 96 we...