anti-Maus p65 Antikörper für Chromatin Immunoprecipitation

Recommended p65 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antikörper
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Epitop
Center
137
72
66
58
51
47
43
40
39
36
33
32
25
20
18
17
16
15
15
13
13
13
12
11
10
9
9
8
7
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
1207
676
519
73
59
55
31
30
28
25
15
8
8
6
5
4
2
1
1
Wirt
Kaninchen
1036
147
17
16
11
1
Klonalität
Polyklonal
Konjugat
Dieser p65 Antikörper ist unkonjugiert
34
34
26
21
19
18
16
16
13
13
13
9
9
9
9
9
9
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Applikation
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
880
429
357
268
223
149
118
87
84
28
22
19
18
11
10
6
6
6
5
4
4
2
2
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN2855287
357,24 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2706683 ChIP IC IF IHC WB Rabbit acLys310 Anmelden zum Anzeigen Polyclonal
1 ABIN2578783 ChIP ICC IF IHC (p) WB Rabbit acLys310 Anmelden zum Anzeigen Polyclonal
1 ABIN1982798 ChIP WB Sheep IgG AA 456-551 Anmelden zum Anzeigen Polyclonal
1 ABIN3071405 ChIP IF/ICC IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal

Ähnliche anti-p65 Antikörper

Applikation / Reaktivität Maus
Chromatin Immunoprecipitation (ChIP) 5 Antikörper
Cytometry by Time of Flight (CyTOF) 4 Antikörper
Electrophoretic Mobility-Shift Assay (EMSA) 2 Antikörper
ELISA 131 Antikörper
Enzyme Immunoassay (EIA) 14 Antikörper
Flow Cytometry (FACS) 53 Antikörper
Gel Shift (GS) 6 Antikörper
Immunochromatography (IC) 6 Antikörper
Immunocytochemistry (ICC) 59 Antikörper
Immunofluorescence (IF) 123 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 13 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 93 Antikörper
Immunohistochemistry (IHC) 295 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 185 Antikörper
Immunoprecipitation (IP) 100 Antikörper
Microarray (MA) 1 Antikörper
Proximity Ligation Assay (PLA) 2 Antikörper

General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antikörper
Epitop Center
(137), (72), (66), (58), (51), (47), (43), (40), (39), (36), (33), (32), (25), (20), (18), (17), (16), (15), (15), (13), (13), (13), (12), (11), (10), (9), (9), (8), (7), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(1207), (676), (519), (73), (59), (55), (31), (30), (28), (25), (15), (8), (8), (6), (5), (4), (2), (1), (1)
Wirt Kaninchen
(1036), (147), (17), (16), (11), (1)
Klonalität
Polyklonal
Konjugat Dieser p65 Antikörper ist unkonjugiert
(34), (34), (26), (21), (19), (18), (16), (16), (13), (13), (13), (9), (9), (9), (9), (9), (9), (8), (8), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Applikation Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(880), (429), (357), (268), (223), (149), (118), (87), (84), (28), (22), (19), (18), (11), (10), (6), (6), (6), (5), (4), (4), (2), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p65 Antikörper

Target Details p65 Anwendungsinformationen Handhabung Bilder
Kreuzreaktivität Rind (Kuh), Schwein, Ratte (Rattus)
Kreuzreaktivität (Details) Pig (98 %), Rat (99 %), Bovine (98 %)
Produktmerkmale Rabbit Polyclonal antibody to NFkB p65 (v-rel reticuloendotheliosis viral oncogene homolog A (avian))
NFkB p65 antibody
Reinigung Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.
Isotyp IgG

Target Details p65

Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung NFkB p65 (NFkBP65 Antibody Abstract)
Hintergrund NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]

Cellular Localization: Nucleus , Cytoplasm
Molekulargewicht 60 kDa
Gen-ID 5970
Forschungsgebiet Atherosclerosis, Alzheimer's Disease, Apoptosis/Necrosis, Transcription Factors, Innate Immunity
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-p65 Antikörper Target Details p65 Handhabung Bilder zurück nach oben
Applikationshinweise Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*
Kommentare

Positive Control: A431 , HepG2 , human NFkB p65-transfected 293T

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1.03 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Konservierungsmittel ProClin
Vorsichtsmaßnahmen This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (Center) (ABIN2855287) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde...
Western Blotting (WB) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (Center) (ABIN2855287) WB Image NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-tra...
Immunohistochemistry (IHC) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (Center) (ABIN2855287) IHC-P Image NFkB p65 antibody detects NFkB p65 protein at cytosol and nucleus on huma...
Western Blotting (WB) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (Center) (ABIN2855287) WB Image Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the...
Immunohistochemistry (IHC) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (Center) (ABIN2855287) IHC-P Image NFkB p65 antibody detects NFkB p65 protein at cytosol on mouse heart by i...
Western Blotting (WB) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (Center) (ABIN2855287) WB Image Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) wer...