anti-Human p65 Antikörper für Electrophoretic Mobility-Shift Assay

Recommended p65 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antikörper
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Epitop
N-Term, NLS Domain
129
85
68
64
50
45
41
38
38
38
27
26
25
23
18
18
17
16
15
15
14
13
13
12
11
9
8
7
6
6
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
1169
678
521
72
56
55
30
25
25
23
13
8
8
6
4
2
1
1
Wirt
Kaninchen
1008
140
25
11
1
1
Klonalität
Polyklonal
Konjugat
Dieser p65 Antikörper ist unkonjugiert
30
28
22
21
20
19
16
14
14
14
13
10
10
8
8
8
8
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Applikation
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
844
443
324
260
206
163
107
105
89
28
18
18
15
12
12
8
6
6
5
4
4
2
2
2
1
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN5556664
$ 411.18
Zzgl. Versandkosten $45.00
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General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antikörper
Epitop N-Term, NLS Domain
(129), (85), (68), (64), (50), (45), (41), (38), (38), (38), (27), (26), (25), (23), (18), (18), (17), (16), (15), (15), (14), (13), (13), (12), (11), (9), (8), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(1169), (678), (521), (72), (56), (55), (30), (25), (25), (23), (13), (8), (8), (6), (4), (2), (1), (1)
Wirt Kaninchen
(1008), (140), (25), (11), (1), (1)
Klonalität
Polyklonal
Konjugat Dieser p65 Antikörper ist unkonjugiert
(30), (28), (22), (21), (20), (19), (16), (14), (14), (14), (13), (10), (10), (8), (8), (8), (8), (8), (8), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Applikation Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(844), (443), (324), (260), (206), (163), (107), (105), (89), (28), (18), (18), (15), (12), (12), (8), (6), (6), (5), (4), (4), (2), (2), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p65 Antikörper

Target Details p65 Anwendungsinformationen Handhabung Bilder
Sequenz Peptide Sequence: R-I-E-E-K-R-K-R-T-Y-E-T
Spezifität This affinity purified antibody is directed against the nuclear localization sequence (NLS) NLS of human p65 and is useful in determining its presence in various assays. The epitope recognized overlaps the NLS of the p65 subunit of the NFκB heterodimer. Therefore, the antibody selectively binds to the activated form of NFκB. Anti-NFκB p65 NLS may react non-specifically with other proteins.
Produktmerkmale Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)
Reinigung Affinity Purified
Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkappaB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Target Details p65

Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung RELA / NF-kB p65 (NFkBP65 Antibody Abstract)
Hintergrund NFκB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFκB bound to IκB. NFκB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFκB subunit p65, similar to p50/p65 heterodimers. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IκB-α. IκB-α binds to the p65 subunit, preventing nuclear localization and DNA binding. IκB- α binding masks the NFκB nuclear localisation signal (NLS). A broad range of external stimuli lead to activation of NFκB and set off signalling cascades that ultimately converge on the IκB kinase (IKK) complex. Activated IKK specifically and directly phosphorylates IκB-α and this phophorylation event targets IκB-α for degradation. As a consequence, NFκB NLS is uncovered and nuclear translocation occurs.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65
Gen-ID 5970
NCBI Accession NP_001138610
UniProt Q04206
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-p65 Antikörper Target Details p65 Handhabung Bilder zurück nach oben
Applikationshinweise Suitable for Immunohistochemistry, Immunoblotting, ELISA and supershift assays. Thisproduct was assayed by Immunoblot and found to be reactive against NFκB p65 NLS at adilution of 1: 2,000 followed by reaction with Peroxidase conjugated Affinity Purified
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Bilder zurück nach oben
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.