anti-Human p65 Antikörper für Control Peptide

Recommended p65 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antikörper
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Epitop
C-Term
136
72
66
58
51
47
43
40
39
36
33
32
25
21
18
17
16
15
15
13
13
13
12
11
10
9
9
8
7
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
1207
677
519
73
59
55
31
30
28
25
15
8
8
6
5
4
2
1
1
Wirt
Maus
1037
146
17
16
11
1
Klonalität
Monoklonal
Konjugat
Dieser p65 Antikörper ist unkonjugiert
34
34
26
21
19
18
16
16
13
13
13
9
9
9
9
9
9
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Applikation
Control Peptide (CP), Western Blotting (WB)
880
429
357
269
224
149
118
88
84
28
22
19
18
11
11
6
6
6
5
4
4
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN964681
157,14 €
Zzgl. Versandkosten 20,00 €, Trockeneispauschale 20,00 € sowie MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN964680 CP WB Mouse IgG2a kappa C-Term Anmelden zum Anzeigen 27F9-G4

Ähnliche anti-p65 Antikörper

Applikation / Reaktivität Human
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Chromatin Immunoprecipitation (ChIP) 10 Antikörper
Control Peptide (CP) 2 Antikörper
Cytometry by Time of Flight (CyTOF) 4 Antikörper
Dot Blot (DB) 28 Antikörper
Electrophoretic Mobility-Shift Assay (EMSA) 6 Antikörper
ELISA 348 Antikörper
Enzyme Immunoassay (EIA) 22 Antikörper
Flow Cytometry (FACS) 84 Antikörper
Gel Shift (GS) 19 Antikörper
Immunochromatography (IC) 10 Antikörper
Immunocytochemistry (ICC) 85 Antikörper
Immunofluorescence (IF) 222 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 17 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 118 Antikörper
Immunohistochemistry (IHC) 423 Antikörper

General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antikörper
Epitop C-Term
(136), (72), (66), (58), (51), (47), (43), (40), (39), (36), (33), (32), (25), (21), (18), (17), (16), (15), (15), (13), (13), (13), (12), (11), (10), (9), (9), (8), (7), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(1207), (677), (519), (73), (59), (55), (31), (30), (28), (25), (15), (8), (8), (6), (5), (4), (2), (1), (1)
Wirt Maus
(1037), (146), (17), (16), (11), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser p65 Antikörper ist unkonjugiert
(34), (34), (26), (21), (19), (18), (16), (16), (13), (13), (13), (9), (9), (9), (9), (9), (9), (8), (8), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Applikation Control Peptide (CP), Western Blotting (WB)
(880), (429), (357), (269), (224), (149), (118), (88), (84), (28), (22), (19), (18), (11), (11), (6), (6), (6), (5), (4), (4), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p65 Antikörper

Target Details p65 Anwendungsinformationen Handhabung Bilder
Spezifität This product was purified from concentrated tissue culture supernate by Protein A chromatography and showed a single band by IEP (immunoelectrophoresis) when tested with anti-mouse antibody. Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.
Produktmerkmale NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. IkappaBalpha-α binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.
Sterilität Sterile filtered
Immunogen NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide
Klon 27F9-G4
Isotyp IgG2a kappa

Target Details p65

Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung NF-kB p65 (NFkBP65 Antibody Abstract)
Hintergrund NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. IkappaBalpha-α binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit
Gen-ID 5970, 223468676
UniProt Q04206
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-p65 Antikörper Target Details p65 Handhabung Bilder zurück nach oben
Applikationshinweise This protein A purified mouse monoclonal antibody is directed against NFkB p65 (Rel A) and a 1:1000 dilution recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately.
Kommentare

Gene Name: RELA

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.
Haltbarkeit 3 months

Bilder

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
 image for anti-p65 Antikörper (Nuclear Factor-KB P65) (C-Term) (ABIN964681) anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody
Western Blotting (WB) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (C-Term) (ABIN964681) anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody (Image 2)
Western Blotting (WB) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (C-Term) (ABIN964681) anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody (Image 3)
 image for anti-p65 Antikörper (Nuclear Factor-KB P65) (C-Term) (ABIN964681) anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody (Image 4)