anti-Human EGF Antikörper für Immunohistochemistry (Frozen Sections)

Recommended EGF Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Epidermal Growth Factor (EGF) Antikörper
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
  • LOC100008808
Reaktivität
Human
339
164
87
19
14
7
4
2
2
1
1
1
Wirt
Kaninchen
308
153
29
24
6
4
Klonalität
Polyklonal
Konjugat
Dieser EGF Antikörper ist konjugiert mit Biotin
46
27
22
12
10
8
7
7
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
353
290
129
91
54
33
30
27
26
25
19
13
10
7
6
5
4
3
2
2
2
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Produktnummer ABIN115992
374,00 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN115991 EIA IHC (fro) IHC (p) WB Biotin Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN115993 EIA Func IHC (fro) IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN115994 EIA Func IHC (fro) IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN3201442 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 609-751 Anmelden zum Anzeigen
1 ABIN4959675 ELISA IHC IHC (fro) IHC (p) WB Mouse IgM Anmelden zum Anzeigen F5

General

Antigen Epidermal Growth Factor (EGF) Antikörper
Reaktivität Human
(339), (164), (87), (19), (14), (7), (4), (2), (2), (1), (1), (1)
Wirt Kaninchen
(308), (153), (29), (24), (6), (4)
Klonalität
Polyklonal
Konjugat Dieser EGF Antikörper ist konjugiert mit Biotin
(46), (27), (22), (12), (10), (8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2)
Applikation Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(353), (290), (129), (91), (54), (33), (30), (27), (26), (25), (19), (13), (10), (7), (6), (5), (4), (3), (2), (2), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-EGF Antikörper

Target Details EGF Anwendungsinformationen Handhabung Bilder
Spezifität This antibody reacts with Epidermal Growth Factor (hEGF).
Reinigung Affinity chromatography.
Immunogen Highly pure (>98%) recombinant hEGF (human EGF).

Target Details EGF

Produktdetails anti-EGF Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung EGF (EGF Antibody Abstract)
Hintergrund Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Gen-ID 1950
NCBI Accession NP_001954
UniProt P01133
Forschungsgebiet Cancer, Growth Factors
Pathways NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Anwendungsinformationen

Produktdetails anti-EGF Antikörper Target Details EGF Handhabung Bilder zurück nach oben
Applikationshinweise ELISA: To detect hEGF by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hEGF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-EGF Antikörper Target Details EGF Anwendungsinformationen Bilder zurück nach oben
Rekonstitution Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Konservierungsmittel Without preservative
Handhabung Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.