anti-Yeast TUBA1B Antikörper für Radioimmunoassay

Recommended TUBA1B Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tubulin, alpha 1B (TUBA1B) Antikörper
  • K-ALPHA-1
  • k-alpha-1
  • tuba
  • tuba1
  • Tuba2
  • RGD1565476
  • TUBA3
  • TUBAT
  • similar to alpha tubulin
  • alpha tubulin
  • tubulin, alpha 1b
  • tubulin, alpha 1B
  • tubulin, alpha 8
  • RGD1560000
  • LOC100009327
  • TUBA1B
  • tuba1b
  • Tuba1b
  • TUBA8
Reaktivität
Vogel, Säugetier, Yeast
44
17
13
8
5
5
3
2
2
2
2
1
1
1
1
1
1
Wirt
Ratte
29
22
1
Klonalität
Monoklonal
Konjugat
Dieser TUBA1B Antikörper ist unkonjugiert
3
2
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
49
24
23
17
14
8
6
6
4
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Dieses Produkt umsonst

Schicken Sie uns Ihren Validierungsvorschlag. Ich möchte dieses Produkt validieren

Mehr erfahren

Produktnummer ABIN120144
605,00 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN121117 EIA IHC (fro) IF IHC (p) IP RIA WB Rat IgG2a Anmelden zum Anzeigen YOL1-34 1

anti-TUBA1B Antikörper für Radioimmunoassay mit den meisten Publikationen

Ähnliche anti-TUBA1B Antikörper

Applikation / Reaktivität Yeast Säugetier
Enzyme Immunoassay (EIA) 2 Antikörper 3 Antikörper
Immunofluorescence (IF) 2 Antikörper 2 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antikörper 2 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antikörper 1 Antikörper
Immunoprecipitation (IP) 2 Antikörper 2 Antikörper
Radioimmunoassay (RIA) 2 Antikörper 2 Antikörper
Western Blotting (WB) 2 Antikörper 3 Antikörper

General

Antigen Tubulin, alpha 1B (TUBA1B) Antikörper
Reaktivität Vogel, Säugetier, Yeast
(44), (17), (13), (8), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Ratte
(29), (22), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser TUBA1B Antikörper ist unkonjugiert
(3), (2), (1)
Applikation Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
(49), (24), (23), (17), (14), (8), (6), (6), (4), (1), (1)
Pubmed 6 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TUBA1B Antikörper

Target Details TUBA1B Anwendungsinformationen Handhabung ProductDetails: References for anti-TUBA1B antibody (ABIN120144) Bilder
Spezifität SM2202P recognises the alpha subunit of Tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognised by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized Actin (5).
Reinigung Affinity Chromatography on Protein G
Immunogen Yeast Tubulin. Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.
Klon YL1-2
Isotyp IgG2a

Target Details TUBA1B

Produktdetails anti-TUBA1B Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-TUBA1B antibody (ABIN120144) Bilder zurück nach oben
Antigen
Andere Bezeichnung alpha Tubulin / TUBA1B (TUBA1B Antibody Abstract)
Hintergrund Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain
Gen-ID 10376
NCBI Accession NP_006073
UniProt P68363
Pathways Microtubule Dynamics, M Phase

Anwendungsinformationen

Produktdetails anti-TUBA1B Antikörper Target Details TUBA1B Handhabung ProductDetails: References for anti-TUBA1B antibody (ABIN120144) Bilder zurück nach oben
Applikationshinweise Immunohistochemistry on frozen sections. ELISA (1/100-1/1000, routinely tested in ELISA on Tubulin) (3). Immunoprecipitation. Radioimmunoassay. Clone YL1/2 has also been reported to work in western blotting (3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TUBA1B Antikörper Target Details TUBA1B Anwendungsinformationen ProductDetails: References for anti-TUBA1B antibody (ABIN120144) Bilder zurück nach oben
Konzentration 1.0 mg/mL
Buffer Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-TUBA1B antibody (ABIN120144)

Produktdetails anti-TUBA1B Antikörper Target Details TUBA1B Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Wallace, Durgan, Jin, Hall: "Cdc42 regulates apical junction formation in human bronchial epithelial cells through PAK4 and Par6B." in: Molecular biology of the cell, Vol. 21, Issue 17, pp. 2996-3006, 2010

Skinner, Bradley, Brown, Johnson, Rhodes, Stammers, Lowe: "Use of the Glu-Glu-Phe C-terminal epitope for rapid purification of the catalytic domain of normal and mutant ras GTPase-activating proteins." in: The Journal of biological chemistry, Vol. 266, Issue 22, pp. 14163-6, 1991

Burns: "Cytoskeleton. Tubulin's terminal tyrosine." in: Nature, Vol. 327, Issue 6118, pp. 103-4, 1987

Wehland, Schröder, Weber: "Amino acid sequence requirements in the epitope recognized by the alpha-tubulin-specific rat monoclonal antibody YL 1/2." in: The EMBO journal, Vol. 3, Issue 6, pp. 1295-300, 1984

Wehland, Willingham, Sandoval: "A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo." in: The Journal of cell biology, Vol. 97, Issue 5 Pt 1, pp. 1467-75, 1983

Kilmartin, Wright, Milstein: "Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line." in: The Journal of cell biology, Vol. 93, Issue 3, pp. 576-82, 1982