anti-Alle Spezies TUBA1B Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended TUBA1B Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tubulin, alpha 1B (TUBA1B) Antikörper
  • K-ALPHA-1
  • k-alpha-1
  • tuba
  • tuba1
  • Tuba2
  • RGD1565476
  • TUBA3
  • TUBAT
  • similar to alpha tubulin
  • alpha tubulin
  • tubulin, alpha 1b
  • tubulin, alpha 1B
  • tubulin, alpha 8
  • RGD1560000
  • LOC100009327
  • TUBA1B
  • tuba1b
  • Tuba1b
  • TUBA8
Reaktivität
Alle Spezies
44
17
13
7
5
5
3
3
3
2
2
2
1
1
1
1
1
Wirt
Maus
28
22
2
Klonalität
Monoklonal
Konjugat
Dieser TUBA1B Antikörper ist unkonjugiert
3
2
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
49
24
22
17
14
8
6
6
4
2
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Produktnummer ABIN112505
264,00 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN112504 IF IHC (p) FITC Mouse IgG1 Anmelden zum Anzeigen TU-01 4
1 ABIN301997 ICC IHC (p) WB Biotin Mouse IgG1 AA 65-97, N-Term Anmelden zum Anzeigen TU-01 13
1 ABIN400675 EIA IF IHC (p) IP WB Mouse IgM Anmelden zum Anzeigen TU-16
1 ABIN93892 ICC IHC (p) WB FITC Mouse IgG1 AA 65-97, N-Term Anmelden zum Anzeigen TU-01 14
1 ABIN93894 ICC IHC (p) IP ELISA WB Mouse IgM Anmelden zum Anzeigen TU-16 3
1 ABIN120542 EIA IHC (fro) IF IHC (p) IP WB Mouse IgM Anmelden zum Anzeigen TU-16

anti-TUBA1B Antikörper für Immunohistochemistry (Paraffin-embedded Sections) mit den meisten Publikationen

Ähnliche anti-TUBA1B Antikörper

Applikation / Reaktivität Alle Spezies
ELISA 1 Antikörper
Enzyme Immunoassay (EIA) 3 Antikörper
Immunocytochemistry (ICC) 3 Antikörper
Immunofluorescence (IF) 4 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antikörper
Immunoprecipitation (IP) 4 Antikörper
Western Blotting (WB) 7 Antikörper

General

Antigen Tubulin, alpha 1B (TUBA1B) Antikörper
Reaktivität Alle Spezies
(44), (17), (13), (7), (5), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Wirt Maus
(28), (22), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser TUBA1B Antikörper ist unkonjugiert
(3), (2), (1)
Applikation Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(49), (24), (22), (17), (14), (8), (6), (6), (4), (2), (1)
Pubmed 10 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TUBA1B Antikörper

Target Details TUBA1B Anwendungsinformationen Handhabung ProductDetails: References for anti-TUBA1B antibody (ABIN112505) Bilder
Spezifität The antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha Tubulin. Reacts with all species (recognized epitope conserved within all species).
Reinigung Precipitation Methods
Immunogen Fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization
Klon TU-01
Isotyp IgG1

Target Details TUBA1B

Produktdetails anti-TUBA1B Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-TUBA1B antibody (ABIN112505) Bilder zurück nach oben
Antigen
Andere Bezeichnung alpha Tubulin / TUBA1B (TUBA1B Antibody Abstract)
Hintergrund The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or morecommonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight about 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structuraldomain.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain
Gen-ID 10376
NCBI Accession NP_006073
UniProt P68363
Pathways Microtubule Dynamics, M Phase

Anwendungsinformationen

Produktdetails anti-TUBA1B Antikörper Target Details TUBA1B Handhabung ProductDetails: References for anti-TUBA1B antibody (ABIN112505) Bilder zurück nach oben
Applikationshinweise Western Blotting (Reducing conditions): Recommended Dilution: 1-2 μg/mL,Incubation Time: 60 min, room temperature. Positive Control: HPB-ALL peripheral blood leukemia cell lysate (incubation 60 min)Porcine brain lysate (incubation 90 min). Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer(1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith reducing Laemmli SDS-PAGE sample buffer. Immunocytochemistry: Recommended Dilution: DY547 conjugate: 2-3 μg/mL, FITC conjugate: 3 μg/mL. Staining technique: fixed and permeabilized cells. Clone TU-01 has also been described to work in Immunohistochemistry, ELISA andImmunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TUBA1B Antikörper Target Details TUBA1B Anwendungsinformationen ProductDetails: References for anti-TUBA1B antibody (ABIN112505) Bilder zurück nach oben
Konzentration 1.0 mg/mL
Buffer PBS, pH ~7.4, 15 mM Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-TUBA1B antibody (ABIN112505)

Produktdetails anti-TUBA1B Antikörper Target Details TUBA1B Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Lukas, Mazna, Valenta, Doubravska, Pospichalova, Vojtechova, Fafilek, Ivanek, Plachy, Novak, Korinek: "Dazap2 modulates transcription driven by the Wnt effector TCF-4." in: Nucleic acids research, Vol. 37, Issue 9, pp. 3007-20, 2009

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, 1997

Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." in: Planta, Vol. 201, Issue 3, pp. 349-58, 1997

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996

Linhartová, Dráber, Dráberová, Viklický: "Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins." in: The Biochemical journal, Vol. 288 ( Pt 3), pp. 919-24, 1993

Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." in: Histochemistry, Vol. 95, Issue 5, pp. 519-24, 1991

Dráber, Dráberová, Linhartová, Viklický: "Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies." in: Journal of cell science, Vol. 92 ( Pt 3), pp. 519-28, 1990

Grimm, Breitling, Little: "Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1." in: Biochimica et biophysica acta, Vol. 914, Issue 1, pp. 83-8, 1987

Dráber, Dráberová, Zicconi, Sellitto, Viklický, Cappuccinelli: "Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin." in: European journal of cell biology, Vol. 41, Issue 1, pp. 82-8, 1987