BlueAQUA Prestained Protein Ladder

Details zu Produkt Nr. ABIN2868520, Anbieter: Anmelden zum Anzeigen
Detektionsbereich
10-180 kDa
Untere Nachweisgrenze
10 kDa
Applikation
SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Verwendungszweck The BlueAQUA Prestained Protein Ladder is a blue protein standard with 11 pre-stained proteins covering a wide range molecular weights from 10 to180 kDa.
Nachweismethode Colorimetric
Produktmerkmale Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer).The BlueAQUA Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Applikationshinweise - 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
- 1.5~2.5 μl per well for general Western transferring.
- Apply more for thicker (> 1.5 mm) or larger gel.
Aufbereitung der Reagenzien

Quality Control: Under suggested conditions, BlueAQUA Prestained Protein Ladder resolves 11 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Approximately 0.1~0.5 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Lagerung -20 °C
Informationen zur Lagerung Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C For long term storage, store at -20°C
Bilder des Herstellers
SDS-PAGE (SDS) image for BlueAQUA Prestained Protein Ladder (ABIN2868520) BlueAQUA Prestained Protein Ladder