BlueRAY Prestained Protein Ladder

Details zu Produkt Nr. ABIN2868254, Anbieter: Anmelden zum Anzeigen
Detektionsbereich
10-180 kDa
Untere Nachweisgrenze
10 kDa
Applikation
SDS-PAGE (SDS), Western Blotting (WB)
Optionen
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Nachweismethode Colorimetric
Applikationshinweise Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2-3 μL per well for general Western transfering. Apply more for thicker (>1.5mm) or larger gel.
5 μL of BlueRAY Prestained Protein Ladder resolves 10 bands in 4-20 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
Kommentare

The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.Do not heat, dilute, add reducing agent before loading.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). Tris-Glycine Bis-Tris 12 % Bis-Tris 10 % 4~20 % MOPS buffer MES buffer
Lagerung RT
Informationen zur Lagerung Store at 25°C for 6 months, Store at -20°C for 24 months
Haltbarkeit 24 months
Bilder des Herstellers
 image for BlueRAY Prestained Protein Ladder (ABIN2868254) Migration patterns of BlueRAY Prestained Protein Ladder in different electrophoresis ...