BLUelf Prestained Protein Ladder

Details zu Produkt Nr. ABIN2868250, Anbieter: Anmelden zum Anzeigen
Detektionsbereich
3.5-245 kDa
Untere Nachweisgrenze
3.5 kDa
Applikation
SDS-PAGE (SDS), Western Blotting (WB)
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Nachweismethode Colorimetric
Applikationshinweise Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μL per well for general Western transfering.
BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.
Kommentare

The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitroce-llulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

Aufbereitung der Reagenzien

Quality Control:
Under suggested conditions, BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM ~3.5 ~5 Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Bis-Tris 10 % Tris-Glycine Urea, and 15 % (v/v) Glycerol). MES buffer 4~20 %
Lagerung RT
Informationen zur Lagerung Store at 25°C for 6 months, Store at -20°C for 24 months
Haltbarkeit 24 months
Bilder des Herstellers
 image for BLUelf Prestained Protein Ladder (ABIN2868250) Migration patterns of BLUelf Prestained Protein Ladder in different electrophoresis c...