Lambda DNA/Hind III Marker

Details zu Produkt Nr. ABIN1540476, Anbieter: Anmelden zum Anzeigen
Detektionsbereich
564-23130 bp
Untere Nachweisgrenze
564 bp
Applikation
Agarose Gel Electrophoresis (AGE)
Optionen
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Verwendungszweck Lambda DNA/ Hind III Marker is valuable for various DNA sizing purposes.
Nachweismethode Fluorometric
Produktmerkmale Lambda DNA/Hind III Marker was digested with restriction enzyme Hind III, followed by phenol/chloroform extraction to inactivate the enzyme and dialyzed in TE buffer. The final DNA concentration is measured at OD260 and compared to other DNA standards for accuracy. This ladder is pre-mixed with loading dye and is ready to use.
7 DNA Fragments (bp): 564, 2027, 2322, 4361, 6557, 9416, and 23130

Range: 564bp-23130bp
Bands: 7
Applikationshinweise Recommended Loading: 6µL/Lane.
Add 6µL ladder directly to each well. You may need more or less than 6µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 6µL/Lane, 8cm, 0.8% Agarose Gel, 0.5xTAE, 7V/cm, 45min.
Kommentare

Do not heat before loading.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Lagerung -20 °C
Informationen zur Lagerung 3 months at room temperature, for long term storage, please keep at -20°C.
Haltbarkeit 3 months
Bilder des Herstellers
Agarose Gel Electrophoresis (AGE) image for Lambda DNA/Hind III Marker (ABIN1540476) Lambda DNA/Hind III Marker