50bp DNA Ladder Plus

Details zu Produkt Nr. ABIN1540466, Anbieter: Anmelden zum Anzeigen
Detektionsbereich
50-1000 bp
Untere Nachweisgrenze
50 bp
Applikation
Agarose Gel Electrophoresis (AGE)
Optionen
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Verwendungszweck 50bp DNA Ladder Plus (Quantitative) is ideal for determining the size of double-stranded DNA from 50 to 1000 base pairs.
Nachweismethode Fluorometric
Produktmerkmale The ladder consists of 13 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 250bp and 500bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 250bp and 500bp are at 40ng. The 250bp and 500bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.
13 DNA Fragments (bp): 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, and 1000
250bp and 500bp Bands: 100ng/5 µL
Other Bands: 40ng/5 µL
Range: 50bp-1kb
Bands: 13
Applikationshinweise Recommended Loading: 5 µL/Lane.
Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 5 µL/Lane, 8 cm 3 % Agarose Gel, 0.5×TBE, 5V/cm, 1h.
Kommentare

Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Lagerung -20 °C
Informationen zur Lagerung 1 year at room temperature, for long term storage, please keep at -20°C.
Haltbarkeit 12 months
Bilder des Herstellers
Agarose Gel Electrophoresis (AGE) image for 50bp DNA Ladder Plus (ABIN1540466) 50bp DNA Ladder Plus