20bp DNA Ladder

Details zu Produkt Nr. ABIN1540464, Anbieter: Anmelden zum Anzeigen
Detektionsbereich
60-300 bp
Untere Nachweisgrenze
60 bp
Applikation
Agarose Gel Electrophoresis (AGE)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck 20bp Ladder is ideal for determining the size of double-stranded DNA from 60 to 300 base pairs.
Nachweismethode Fluorometric
Produktmerkmale The ladder consists of 13 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.
13 DNA Fragments (bp): 60, 80, 100, 120, 140, 160, 180, 200, 220, 240, 260, 280, and 300
100bp and 200bp Bands: 100ng/5 µL
Other Bands: 40ng/5 µL
Range: 60bp-300bp
Bands: 13
Applikationshinweise Recommended Loading: 5 µL/Lane.
Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 5 µL/Lane, 8cm, 4% Agarose Gel, 1×TBE, 5V/cm, 1h.
Kommentare

Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Lagerung -20 °C
Informationen zur Lagerung 1 year at room temperature, for long term storage, please keep at -20°C.
Haltbarkeit 12 months
Bilder des Herstellers
Agarose Gel Electrophoresis (AGE) image for 20bp DNA Ladder (ABIN1540464) 20bp DNA Ladder