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LMW DNA Ladder

25-700 bp Fluorometric
Produktnummer ABIN1540462
  • Nachweismethode
    Fluorometric
    Detektionsbereich
    25-700 bp
    Untere Nachweisgrenze
    25 bp
    Applikation
    Agarose Gel Electrophoresis (AGE)
    Verwendungszweck
    LMW DNA Ladder is ideal for determining the size of double-stranded DNA from 25 to 700 base pairs.
    Produktmerkmale
    The ladder consists of 10 linear double-stranded DNA fragments, chromatography- purified. The intensity of the 100bp and 300bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 100bp and 300bp are at 40ng. The 100bp and 300bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.
    10 DNA Fragments (bp): 25, 50, 75, 100, 150, 200, 300, 400, 500, and 700
    100bp and 300bp Bands: 100ng/5 µL
    Other Bands: 40ng/5 µL
    Range: 25bp-700bp
    Bands: 10
  • Applikationshinweise
    Recommended Loading: 5 µL/Lane.
    Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
    Recommended Loading: 5 µL/Lane, 8cm, 3 % Agarose Gel, 0.5×TBE, 5V/cm, 1h.
    Kommentare

    Do not heat before loading.
    For quantification, adjust the concentration of the sample to equalize.
    Visualize DNA by staining with ethidium bromide or with SYBR Green I.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Lagerung
    -20 °C
    Informationen zur Lagerung
    1 year at room temperature, for long term storage, please keep at -20°C.
    Haltbarkeit
    12 months
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