anti-Human RAF1 Antikörper für Immunofluorescence (Paraffin-embedded Sections)

Recommended RAF1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antikörper
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitop
AA 25-60
73
72
47
40
39
37
28
28
28
27
22
22
22
20
19
18
17
15
15
15
15
14
14
14
10
8
8
7
6
6
6
6
6
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
808
410
385
25
23
16
16
13
8
8
8
5
4
4
2
2
1
1
1
Wirt
Kaninchen
769
46
12
6
2
Klonalität
Polyklonal
Konjugat
Dieser RAF1 Antikörper ist unkonjugiert
44
44
43
29
29
29
13
13
12
12
12
12
12
12
12
12
12
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
489
348
202
172
155
103
50
34
19
18
17
7
2
2
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN733208
315,94 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN712706 IF (p) IHC (p) WB Rabbit IgG AA 325-375, pTyr341 Anmelden zum Anzeigen Polyclonal 1
1 ABIN733628 IF (p) IHC (p) WB Rabbit IgG Tyr340, pSer338 Anmelden zum Anzeigen Polyclonal
1 ABIN712736 IF (p) IHC (p) WB Rabbit IgG AA 330-345, pSer339 Anmelden zum Anzeigen Polyclonal
1 ABIN745343 IF (p) IHC (p) WB Rabbit IgG pSer289 Anmelden zum Anzeigen Polyclonal
1 ABIN879528 IF (p) Alexa Fluor 350 Rabbit IgG Tyr340, pSer338 Anmelden zum Anzeigen Polyclonal
1 ABIN879527 IF (p) Alexa Fluor 488 Rabbit IgG Tyr340, pSer338 Anmelden zum Anzeigen Polyclonal
1 ABIN879529 IF (p) Alexa Fluor 555 Rabbit IgG Tyr340, pSer338 Anmelden zum Anzeigen Polyclonal
1 ABIN879530 IF (p) Alexa Fluor 647 Rabbit IgG Tyr340, pSer338 Anmelden zum Anzeigen Polyclonal
1 ABIN733631 IF (p) Cy3 Rabbit IgG Tyr340, pSer338 Anmelden zum Anzeigen Polyclonal
1 ABIN733632 IF (p) Cy5 Rabbit IgG Tyr340, pSer338 Anmelden zum Anzeigen Polyclonal
1 ABIN733633 IF (p) Cy5.5 Rabbit IgG Tyr340, pSer338 Anmelden zum Anzeigen Polyclonal
1 ABIN733634 IF (p) Cy7 Rabbit IgG Tyr340, pSer338 Anmelden zum Anzeigen Polyclonal
1 ABIN733635 IF (p) FITC Rabbit IgG Tyr340, pSer338 Anmelden zum Anzeigen Polyclonal
1 ABIN2810550 IF (p) Alexa Fluor 594 Rabbit IgG AA 310-360, Tyr340, pSer338 Anmelden zum Anzeigen Polyclonal
1 ABIN875647 IF (p) Alexa Fluor 350 Rabbit IgG AA 330-345, pSer339 Anmelden zum Anzeigen Polyclonal
1 ABIN875646 IF (p) Alexa Fluor 488 Rabbit IgG AA 330-345, pSer339 Anmelden zum Anzeigen Polyclonal
1 ABIN875648 IF (p) Alexa Fluor 555 Rabbit IgG AA 330-345, pSer339 Anmelden zum Anzeigen Polyclonal
1 ABIN875649 IF (p) Alexa Fluor 647 Rabbit IgG AA 330-345, pSer339 Anmelden zum Anzeigen Polyclonal
1 ABIN712739 IF (p) Cy3 Rabbit IgG AA 330-345, pSer339 Anmelden zum Anzeigen Polyclonal
1 ABIN712740 IF (p) Cy5 Rabbit IgG AA 330-345, pSer339 Anmelden zum Anzeigen Polyclonal

anti-RAF1 Antikörper für Immunofluorescence (Paraffin-embedded Sections) mit den meisten Publikationen

Ähnliche anti-RAF1 Antikörper

Applikation / Reaktivität Ratte (Rattus) Maus
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ELISA 90 Antikörper 96 Antikörper
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Immunochromatography (IC) 2 Antikörper 2 Antikörper
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Immunofluorescence (IF) 23 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 6 Antikörper
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Immunohistochemistry (IHC) 76 Antikörper
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Immunoprecipitation (IP) 11 Antikörper
RNA Interference (RNAi) 1 Antikörper
Western Blotting (WB) 234 Antikörper
Flow Cytometry (FACS) 11 Antikörper

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antikörper
Epitop AA 25-60
(73), (72), (47), (40), (39), (37), (28), (28), (28), (27), (22), (22), (22), (20), (19), (18), (17), (15), (15), (15), (15), (14), (14), (14), (10), (8), (8), (7), (6), (6), (6), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(808), (410), (385), (25), (23), (16), (16), (13), (8), (8), (8), (5), (4), (4), (2), (2), (1), (1), (1)
Wirt Kaninchen
(769), (46), (12), (6), (2)
Klonalität
Polyklonal
Konjugat Dieser RAF1 Antikörper ist unkonjugiert
(44), (44), (43), (29), (29), (29), (13), (13), (12), (12), (12), (12), (12), (12), (12), (12), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(489), (348), (202), (172), (155), (103), (50), (34), (19), (18), (17), (7), (2), (2), (2), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-RAF1 Antikörper

Target Details RAF1 Anwendungsinformationen Handhabung ProductDetails: References for anti-RAF1 antibody (ABIN733208) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human c Raf
Isotyp IgG

Target Details RAF1

Produktdetails anti-RAF1 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-RAF1 antibody (ABIN733208) Bilder zurück nach oben
Antigen
Andere Bezeichnung C-Raf/Raf1 (RAF1 Antibody Abstract)
Hintergrund

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Subcellular location: Cytoplasm, Nucleus, Mitochondrion, Cell membrane

Synonyms: NS5, CRAF, Raf-1, c-Raf, CMD1NN, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, RAF1, RAF

Gen-ID 5894
UniProt P04049
Pathways MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor

Anwendungsinformationen

Produktdetails anti-RAF1 Antikörper Target Details RAF1 Handhabung ProductDetails: References for anti-RAF1 antibody (ABIN733208) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-RAF1 Antikörper Target Details RAF1 Anwendungsinformationen ProductDetails: References for anti-RAF1 antibody (ABIN733208) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

ProductDetails: References for anti-RAF1 antibody (ABIN733208)

Produktdetails anti-RAF1 Antikörper Target Details RAF1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Wang, Fu, Jiang, Yu, Wang, Han, Liu, Wang: "Bone marrow mononuclear cell transplantation promotes therapeutic angiogenesis via upregulation of the VEGF-VEGFR2 signaling pathway in a rat model of vascular dementia." in: Behavioural brain research, Vol. 265, pp. 171-80, 2014

Bilder

Produktdetails anti-RAF1 Antikörper Target Details RAF1 Anwendungsinformationen Handhabung ProductDetails: References for anti-RAF1 antibody (ABIN733208) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-RAF1 Antikörper (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (AA 25-60) (ABIN733208) Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-c-RafRaf1 ...
Immunohistochemistry (IHC) image for anti-RAF1 Antikörper (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (AA 25-60) (ABIN733208) Images provided the Independent Validation Program (badge number 29651)Formalin-fixed...
'Independent Validation' Siegel
Chargennummer 130909
Validierte Anwendung Immunohistochemistry
Positivkontrolle Human colon villus epithelial cells
Negativkontrolle Human liver
Bewertung Raf1 exhibits uniformly low expression across all human tissues; higher expression found in colon, lower in liver. Tissue-specific expression is detected in colon, with expected low level expression in liver.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: Human colon tissue stained with antibody
  • Negative control: Human liver tissue stained with antibody
  • Isotype control: Human colon tissue stained with isotype control
  • Secondary only control: Human colon tissue stained with secondary antibody only
Protokoll
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Anmerkungen
  • Deviations from protocol/procedure supplied by manufacturer:
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validierungsbilder
Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 25-60) antibody (ABIN733208) Figure 1: Human colon tissue stained with anti-RAF1 (brown) and counterstained with h...
Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 25-60) antibody (ABIN733208) Figure 2: Human liver tissue stained with anti-RAF1 (brown) and counterstained with h...
Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 25-60) antibody (ABIN733208) Figure 3: Human colon tissue stained with isotype control antibody (brown) and counte...
Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 25-60) antibody (ABIN733208) Figure 4: Human colon tissue stained with secondary atnibody only (brown) and counter...