anti-Ratte (Rattus) p38 Antikörper für Intracellular Staining

Recommended p38 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
MAP kinase p38 (p38) Antikörper
  • CSBP
  • CSBP1
  • CSBP2
  • CSPB1
  • EXIP
  • Mxi2
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • p38
  • p38ALPHA
  • mitogen-activated protein kinase 14
  • MAPK14
Epitop
pThr180
79
63
33
20
15
15
12
10
9
8
8
6
5
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
367
265
262
48
30
29
28
26
26
25
21
21
15
15
12
7
5
3
2
1
1
1
Wirt
Maus
277
93
Klonalität
Monoklonal
Konjugat
Dieser p38 Antikörper ist unkonjugiert
10
10
9
7
7
7
6
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
BioImaging (BI), Intracellular Staining (ICS), Western Blotting (WB)
305
159
153
94
67
55
46
29
28
18
13
9
7
6
6
1
1
1
1
1
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN968804
341,03 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN968803 BI ICS WB Mouse IgG1, kappa pThr180 Anmelden zum Anzeigen 36-p38 3
1 ABIN1177120 ICS Pacific Blue Mouse IgG1, kappa pThr180 Anmelden zum Anzeigen 36-p38 3
1 ABIN1177121 ICS PE-Cy7 Mouse IgG1, kappa pThr180 Anmelden zum Anzeigen 36-p38 3
1 ABIN1177119 ICS PerCP-Cy5.5 Mouse IgG1, kappa pThr180 Anmelden zum Anzeigen 36-p38 3
1 ABIN1177122 ICS PE Mouse IgG1, kappa pThr180 Anmelden zum Anzeigen 36-p38 5
1 ABIN1177124 ICS Alexa Fluor 488 Mouse IgG1, kappa pThr180 Anmelden zum Anzeigen 36-p38 3
1 ABIN1177117 ICS PE Mouse IgG1, kappa pThr180 Anmelden zum Anzeigen 36-p38 3
1 ABIN1177118 ICS Alexa Fluor 647 Mouse IgG1, kappa pThr180 Anmelden zum Anzeigen 36-p38 3
1 ABIN1177123 ICS Alexa Fluor 647 Mouse IgG1, kappa pThr180 Anmelden zum Anzeigen 36-p38

anti-p38 Antikörper für Intracellular Staining mit den meisten Publikationen

Ähnliche anti-p38 Antikörper

Applikation / Reaktivität Ratte (Rattus) Maus
Antibody Array (AA) 18 Antikörper 18 Antikörper
BioImaging (BI) 2 Antikörper 2 Antikörper
ELISA 101 Antikörper 90 Antikörper
Flow Cytometry (FACS) 6 Antikörper 7 Antikörper
Immunochromatography (IC) 4 Antikörper
Immunocytochemistry (ICC) 28 Antikörper
Immunofluorescence (IF) 39 Antikörper
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 6 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 28 Antikörper
Immunohistochemistry (IHC) 117 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antikörper
Immunoprecipitation (IP) 84 Antikörper
Intracellular Staining (ICS) 10 Antikörper
Western Blotting (WB) 215 Antikörper

General

Antigen MAP kinase p38 (p38) Antikörper
Epitop pThr180
(79), (63), (33), (20), (15), (15), (12), (10), (9), (8), (8), (6), (5), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(367), (265), (262), (48), (30), (29), (28), (26), (26), (25), (21), (21), (15), (15), (12), (7), (5), (3), (2), (1), (1), (1)
Wirt Maus
(277), (93)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser p38 Antikörper ist unkonjugiert
(10), (10), (9), (7), (7), (7), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Applikation BioImaging (BI), Intracellular Staining (ICS), Western Blotting (WB)
(305), (159), (153), (94), (67), (55), (46), (29), (28), (18), (13), (9), (7), (6), (6), (1), (1), (1), (1), (1), (1)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p38 Antikörper

Target Details p38 Anwendungsinformationen Handhabung ProductDetails: References for anti-p38 antibody (ABIN968804) Bilder
Kreuzreaktivität Maus, Ratte (Rattus)
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human p38 MAPK (pT180/pY182) Peptide
Klon 36-p38
Isotyp IgG1, kappa

Target Details p38

Produktdetails anti-p38 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-p38 antibody (ABIN968804) Bilder zurück nach oben
Antigen
Andere Bezeichnung p38 MAPK (p38 Antibody Abstract)
Hintergrund Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytes results in the production and release of proinflammatory cytokines, such as IL-1 and TNF. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the Mitogen Activated Protein Kinases (MAPKs). MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAPKs include p38alpha (MAPK14), beta (MAPK11), gamma (MAPK12), and delta (MAPK13). These Ser/Thr kinases are activated by dual phosphorylation on threonine (T) and tyrosine (Y) within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP Kinase Kinases, MKK3, MKK4, and MKK6. This leads to the activation of multiple transcription factors (NF-kappaB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes. Thus, p38 MAPKs are central kinases in multiple signal transduction pathways.
The 36/p38 (pT180/pY182) monoclonal antibody recognizes the conserved dual phosphorylated site pT180/pY182 of p38alpha, beta, gamma, and delta.
Synonyms: MK14, 11, 12, 13, CSBP1, SAPK2, 2A, 3, 4, MX12, ERK-6, ERK5
Molekulargewicht 38-42 kDa
Forschungsgebiet Kinases/Phosphatases
Pathways MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling

Anwendungsinformationen

Produktdetails anti-p38 Antikörper Target Details p38 Handhabung ProductDetails: References for anti-p38 antibody (ABIN968804) Bilder zurück nach oben
Applikationshinweise Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate 1 hour.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark 1 hour.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Kommentare

Related Products: ABIN968535, ABIN968632, ABIN968769

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p38 Antikörper Target Details p38 Anwendungsinformationen ProductDetails: References for anti-p38 antibody (ABIN968804) Bilder zurück nach oben
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20°C.

ProductDetails: References for anti-p38 antibody (ABIN968804)

Produktdetails anti-p38 Antikörper Target Details p38 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Winston, Chan, Johnson, Riches: "Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, 1997

Brunet, Pouysségur: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." in: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, 1996

Han, Lee, Bibbs, Ulevitch: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994

Bilder

Produktdetails anti-p38 Antikörper Target Details p38 Anwendungsinformationen Handhabung ProductDetails: References for anti-p38 antibody (ABIN968804) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-p38 Antikörper (MAP kinase p38) (pThr180) (ABIN968804) Western blot analysis for p38 MAPK (pT180/pY182). HeLa cells (Human cervical epithelo...
Immunofluorescence (IF) image for anti-p38 Antikörper (MAP kinase p38) (pThr180) (ABIN968804) Immunofluorescent staining of HeLa cells. HeLa cells (Human cervical epitheloid carci...
 image for anti-p38 Antikörper (MAP kinase p38) (pThr180) (ABIN968804) Cells exposed to anisomycin for 30 minutes