anti-Human p38 Antikörper für Immunofluorescence (Paraffin-embedded Sections)

Recommended p38 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
MAP kinase p38 (p38) Antikörper
  • CSBP
  • CSBP1
  • CSBP2
  • CSPB1
  • EXIP
  • Mxi2
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • p38
  • p38ALPHA
  • mitogen-activated protein kinase 14
  • MAPK14
Epitop
pThr180, pTyr182, AA 160-200
67
56
31
15
14
10
10
6
5
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus), Ziege
280
222
216
38
22
22
21
20
19
18
18
18
15
14
12
4
1
1
Wirt
Kaninchen
197
85
Klonalität
Polyklonal
Konjugat
Dieser p38 Antikörper ist unkonjugiert
7
7
7
7
6
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
223
161
110
69
61
56
47
27
27
18
13
10
6
6
2
2
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN678668
$ 329.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN671241 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 160-210 Anmelden zum Anzeigen Polyclonal 10
1 ABIN710141 IF (p) IHC (p) WB Rabbit IgG pTyr323 Anmelden zum Anzeigen Polyclonal 5
1 ABIN2804957 IF (cc) IF (p) Alexa Fluor 594 Rabbit IgG AA 160-210 Anmelden zum Anzeigen Polyclonal
1 ABIN2804958 IF (p) Alexa Fluor 594 Rabbit IgG AA 160-200, pThr180, pTyr182 Anmelden zum Anzeigen Polyclonal
1 ABIN678672 IF (p) Cy5 Rabbit IgG pThr180, pTyr182, AA 160-200 Anmelden zum Anzeigen Polyclonal
1 ABIN678674 IF (p) Cy7 Rabbit IgG pThr180, pTyr182, AA 160-200 Anmelden zum Anzeigen Polyclonal
1 ABIN678675 IF (p) FITC Rabbit IgG pThr180, pTyr182, AA 160-200 Anmelden zum Anzeigen Polyclonal
1 ABIN678673 IF (p) Cy5.5 Rabbit IgG pThr180, pTyr182, AA 160-200 Anmelden zum Anzeigen Polyclonal
1 ABIN671244 IF (cc) IF (p) Cy3 Rabbit IgG AA 160-210 Anmelden zum Anzeigen Polyclonal
1 ABIN678671 IF (p) Cy3 Rabbit IgG pThr180, pTyr182, AA 160-200 Anmelden zum Anzeigen Polyclonal
1 ABIN671246 IF (cc) IF (p) Cy5.5 Rabbit IgG AA 160-210 Anmelden zum Anzeigen Polyclonal
1 ABIN671245 IF (cc) IF (p) Cy5 Rabbit IgG AA 160-210 Anmelden zum Anzeigen Polyclonal
1 ABIN671247 IF (cc) IF (p) Cy7 Rabbit IgG AA 160-210 Anmelden zum Anzeigen Polyclonal
1 ABIN671248 IF (cc) IF (p) FITC Rabbit IgG AA 160-210 Anmelden zum Anzeigen Polyclonal
1 ABIN879151 IF (p) Alexa Fluor 555 Rabbit IgG pThr180, pTyr182, AA 160-200 Anmelden zum Anzeigen Polyclonal
1 ABIN879152 IF (p) Alexa Fluor 647 Rabbit IgG pThr180, pTyr182, AA 160-200 Anmelden zum Anzeigen Polyclonal
1 ABIN879153 IF (p) Alexa Fluor 350 Rabbit IgG pThr180, pTyr182, AA 160-200 Anmelden zum Anzeigen Polyclonal
1 ABIN879154 IF (p) Alexa Fluor 488 Rabbit IgG pThr180, pTyr182, AA 160-200 Anmelden zum Anzeigen Polyclonal
1 ABIN909746 IF (cc) IF (p) Alexa Fluor 647 Rabbit IgG AA 160-210 Anmelden zum Anzeigen Polyclonal
1 ABIN909745 IF (cc) IF (p) Alexa Fluor 555 Rabbit IgG AA 160-210 Anmelden zum Anzeigen Polyclonal

General

Antigen MAP kinase p38 (p38) Antikörper
Epitop pThr180, pTyr182, AA 160-200
(67), (56), (31), (15), (14), (10), (10), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus), Ziege
(280), (222), (216), (38), (22), (22), (21), (20), (19), (18), (18), (18), (15), (14), (12), (4), (1), (1)
Wirt Kaninchen
(197), (85)
Klonalität
Polyklonal
Konjugat Dieser p38 Antikörper ist unkonjugiert
(7), (7), (7), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(223), (161), (110), (69), (61), (56), (47), (27), (27), (18), (13), (10), (6), (6), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p38 Antikörper

Target Details p38 Anwendungsinformationen Handhabung Referenzen für anti-p38 Antikörper (ABIN678668) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human p38 MAPK around the phosphorylation site of(Thr180/Tyr182)
Isotyp IgG

Target Details p38

Produktdetails anti-p38 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-p38 Antikörper (ABIN678668) Bilder zurück nach oben
Antigen
Andere Bezeichnung P38 Mapk (p38 Antibody Abstract)
Hintergrund

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Subcellular location: Cytoplasm, Nucleus

Synonyms: RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, MAP kinase p38 alpha, Stress-activated protein kinase 2a, MAPK14

Gen-ID 1432
UniProt Q16539
Forschungsgebiet Kinases/Phosphatases
Pathways MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling

Anwendungsinformationen

Produktdetails anti-p38 Antikörper Target Details p38 Handhabung Referenzen für anti-p38 Antikörper (ABIN678668) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p38 Antikörper Target Details p38 Anwendungsinformationen Referenzen für anti-p38 Antikörper (ABIN678668) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-p38 Antikörper (ABIN678668)

Produktdetails anti-p38 Antikörper Target Details p38 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Iriyama, Hatta, Takei: "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." in: Cancer medicine, Vol. 5, Issue 11, pp. 3223-3234, 2016

Li, Yu, Liu, Zhu, Hua: "Expression of miR-34c in response to overexpression of Boule and Stra8 in dairy goat male germ line stem cells (mGSCs)." in: Cell biochemistry and function, Vol. 31, Issue 4, pp. 281-8, 2013

Bilder

Produktdetails anti-p38 Antikörper Target Details p38 Anwendungsinformationen Handhabung Referenzen für anti-p38 Antikörper (ABIN678668) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-MAP kinase p38 (p38) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Human MCF7 lysates probed with Rabbit Anti-p38 MAPK (Thr180+Tyr182) Polyclonal Antibo...
Western Blotting (WB) image for anti-MAP kinase p38 (p38) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Images provided by the Independent Validation Program (badge 29798).Lane one: HeLa ce...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-MAP kinase p38 (p38) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Images provided the Independent Validation Program (badge number 029662)Formalin-fixe...
'Independent Validation' Siegel
Chargennummer 140106
Validierte Anwendung Immunohistochemistry
Positivkontrolle Human placenta tissue
Negativkontrolle Human tonsil tissue (no literature evidence of phospho-p38 MAPK in tonsil)
Bewertung Signal is detected in positive control tissue and not in negative control tissue.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: Human placenta tissue stained with antibody
  • Negative control: Human tonsil tissue stained with antibody
  • Isotype control: Human placenta tissue stained with isotype control
  • Secondary only control: Human placenta tissue stained with secondary antibody only
Protokoll
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Anmerkungen
  • Deviations from protocol/procedure supplied by manufacturer:
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validierungsbilder
Immunohistochemistry validation image for anti-MAP kinase p38 (p38) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 1: Human placenta tissue stained with anti-phospho-MAPK14 (brown) and counters...
Immunohistochemistry validation image for anti-MAP kinase p38 (p38) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 2: Human tonsil tissue stained with anti-phospho-MAPK14 (brown) and countersta...
Immunohistochemistry validation image for anti-MAP kinase p38 (p38) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 3: Human placenta tissue stained with isotype control antibody (brown) and cou...
Immunohistochemistry validation image for anti-MAP kinase p38 (p38) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 4: Human placenta tissue stained with secondary antibody only (brown) and coun...