anti-Human MAP2K5 Antikörper für Western Blotting

Recommended MAP2K5 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antikörper
  • Mek5
  • HsT17454
  • MAPKK5
  • MEK5
  • PRKMK5
  • AI324775
  • AI428457
  • Mapkk5
  • Prkmk5
  • fb73f02
  • wu:fb73f02
  • zgc:172137
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5
  • ATMAP2K_ALPHA
  • ATMEK5
  • ATMKK5
  • MAP KINASE KINASE 5
  • MAP kinase kinase 5
  • MAP2K_A
  • mitogen activated protein kinase kinase 5
  • mitogen-activated protein kinase kinase 5
  • Map2k5
  • MAP2K5
  • map2k5
  • MKK5
Epitop
AA 380-430
33
30
15
15
15
14
14
11
11
9
6
6
6
5
4
4
3
2
2
2
2
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
168
88
81
11
7
7
6
4
3
3
3
3
2
2
2
2
1
1
Wirt
Kaninchen
162
16
Klonalität
Polyklonal
Konjugat
Dieser MAP2K5 Antikörper ist unkonjugiert
9
9
9
5
5
5
5
5
5
5
5
5
5
4
4
4
4
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
110
63
47
28
24
22
4
3
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN754183
315,94 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.3471746 ABIN392401 IHC (p) WB Rabbit Ig AA 127-156, Ser149 Anmelden zum Anzeigen Polyclonal 1
3.3471746 ABIN393416 IF WB Mouse IgG1 kappa Met11 Anmelden zum Anzeigen 3E10 5
3.3471746 ABIN968272 IF WB Mouse IgG1 AA 13-188 Anmelden zum Anzeigen LPR-01 2
3.3471746 ABIN968273 IF WB Mouse IgG1 AA 13-188 Anmelden zum Anzeigen 21-MEK5 2
3.3471746 ABIN2783608 WB Rabbit N-Term Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN658075 WB Rabbit Ig AA 385-412, C-Term Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN126841 WB Mouse IgG1 Anmelden zum Anzeigen 14B5
3.3471746 ABIN801978 IF (p) IHC (p) WB Rabbit IgG pSer137 Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN1870358 WB Rabbit IgG pSer311, pThr315 Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN4333509 IHC IHC (p) WB Rabbit IgG Center Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN3198202 IC IF WB Rabbit Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN3181576 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN4333505 IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN4333506 ICC IF IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN2563757 IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN519261 IF WB Mouse AA 1-448, full length Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN562429 IF ELISA WB Mouse IgG1 kappa AA 1-448, full length Anmelden zum Anzeigen M1-E6
3.3471746 ABIN562434 IF ELISA WB Mouse IgG1 kappa AA 1-120, partial Anmelden zum Anzeigen 3E10
3.3471746 ABIN928258 WB Rabbit N-Term Anmelden zum Anzeigen Polyclonal
3.3471746 ABIN3020325 WB Rabbit pSer311, pThr315 Anmelden zum Anzeigen Polyclonal

anti-MAP2K5 Antikörper für Western Blotting mit den meisten Publikationen

Ähnliche anti-MAP2K5 Antikörper

Applikation / Reaktivität Ratte (Rattus) Maus
ELISA 7 Antikörper 13 Antikörper
Immunochromatography (IC) 1 Antikörper 1 Antikörper
Immunofluorescence (IF) 6 Antikörper 6 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 2 Antikörper 2 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 38 Antikörper 38 Antikörper
Immunohistochemistry (IHC) 10 Antikörper 9 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antikörper 11 Antikörper
Immunoprecipitation (IP) 1 Antikörper 1 Antikörper
Western Blotting (WB) 46 Antikörper 52 Antikörper

General

Antigen Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antikörper
Epitop AA 380-430
(33), (30), (15), (15), (15), (14), (14), (11), (11), (9), (6), (6), (6), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(168), (88), (81), (11), (7), (7), (6), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Wirt Kaninchen
(162), (16)
Klonalität
Polyklonal
Konjugat Dieser MAP2K5 Antikörper ist unkonjugiert
(9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(110), (63), (47), (28), (24), (22), (4), (3), (2), (1), (1), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MAP2K5 Antikörper

Target Details MAP2K5 Anwendungsinformationen Handhabung ProductDetails: References for anti-MAP2K5 antibody (ABIN754183) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human MEK5
Isotyp IgG

Target Details MAP2K5

Produktdetails anti-MAP2K5 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-MAP2K5 antibody (ABIN754183) Bilder zurück nach oben
Antigen
Andere Bezeichnung Mek5 (MAP2K5 Antibody Abstract)
Hintergrund

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Subcellular location: Cytoplasm, Nucleus

Synonyms: MEK5, MAPKK5, Dual specificity mitogen-activated protein kinase kinase 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MAP kinase kinase 5

Gen-ID 5607
UniProt Q13163
Forschungsgebiet Cancer, Kinases/Phosphatases
Pathways MAPK Signalweg, Neurotrophin Signalübertragung

Anwendungsinformationen

Produktdetails anti-MAP2K5 Antikörper Target Details MAP2K5 Handhabung ProductDetails: References for anti-MAP2K5 antibody (ABIN754183) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MAP2K5 Antikörper Target Details MAP2K5 Anwendungsinformationen ProductDetails: References for anti-MAP2K5 antibody (ABIN754183) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

ProductDetails: References for anti-MAP2K5 antibody (ABIN754183)

Produktdetails anti-MAP2K5 Antikörper Target Details MAP2K5 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Chen, Hou, Ye, Chen, Cui, Tian, Li, Liu: "MicroRNA-143 regulates adipogenesis by modulating the MAP2K5-ERK5 signaling." in: Scientific reports, Vol. 4, pp. 3819, 2014

Li, Dong, Wei, Wang, Zhang, Li: "Fatty acid synthase mediates the epithelial-mesenchymal transition of breast cancer cells." in: International journal of biological sciences, Vol. 10, Issue 2, pp. 171-80, 2014

Bilder

Produktdetails anti-MAP2K5 Antikörper Target Details MAP2K5 Anwendungsinformationen Handhabung ProductDetails: References for anti-MAP2K5 antibody (ABIN754183) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-MAP2K5 Antikörper (Mitogen-Activated Protein Kinase Kinase 5) (AA 380-430) (ABIN754183) Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-MEK5/MAP2K5 P...
Immunohistochemistry (IHC) image for anti-MAP2K5 Antikörper (Mitogen-Activated Protein Kinase Kinase 5) (AA 380-430) (ABIN754183) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Western Blotting (WB) image for anti-MAP2K5 Antikörper (Mitogen-Activated Protein Kinase Kinase 5) (AA 380-430) (ABIN754183) Mouse heart lysates probed with Rabbit Anti-MEK5 Antibody, Unconjugated at 1:5000 fo...
'Independent Validation' Siegel
Chargennummer 120828
Validierte Anwendung Immunohistochemistry
Positivkontrolle Human breast myoepithelial and glandular tissue
Negativkontrolle Human breast adipose tissue
Bewertung Strong signal was detected in positive control sample, with minor background seen in negative control sample.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: Human breast myoepithelial and glandular tissue stained with antibody
  • Negative control: Human breast adipose tissue stained with antibody
  • Isotype control: Human breast myoepithelial and glandular tissue stained with isotype control
  • Secondary only control: Human breast myoepithelial and glandular tissue stained with secondary antibody only
Protokoll
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Anmerkungen
  • Deviations from protocol/procedure supplied by manufacturer (attached).
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validierungsbilder
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 1: Human breast tissue stained with anti-MAP2K5 (brown) and counterstained wit...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 2: Human breast adipose tissue stained with anti-MAP2K5 (brown) and countersta...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 3: Human breast tissue stained with isotype control antibody (brown) and count...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 4: Human breast tissue stained with secondary antibody only (brown) and counte...