anti-Human ERK2 Antikörper für Enzyme Immunoassay

Recommended ERK2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antikörper
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • ATMPK2
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • T30E16.13
  • T30E16_13
  • mitogen-activated protein kinase homolog 2
  • ERK2
  • zERK2
  • MAPK1
  • ERK
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • erk
  • erk2
  • ert1
  • mapk
  • mpk1
  • xp42
  • mapk2
  • prkm1
  • prkm2
  • Xp42
  • mapk1b
  • mapk1
  • mapk1a
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase homolog 2
  • mitogen activated protein kinase 1
  • mitogen-activated protein kinase 1 L homeolog
  • mitogen-activated protein kinase 1 S homeolog
  • LOC9328617
  • MPK1
  • MPK2
  • MAPK1
  • mapk1
  • Mapk1
  • PfMAP1
  • PVX_084965
  • Tsp_01601
  • mapk1.L
  • mapk1.S
Epitop
N-Term
60
21
15
11
9
9
8
8
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
352
147
118
50
30
19
14
11
10
9
8
7
5
5
2
2
2
2
2
1
1
1
Wirt
Maus
169
167
16
8
3
Klonalität
Monoklonal
Konjugat
Dieser ERK2 Antikörper ist unkonjugiert
10
9
8
7
7
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
326
157
94
83
82
63
52
26
22
9
5
3
3
2
2
1
1
1
1
1
1
1
1
Hersteller
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Bilder

Produktnummer ABIN126833
$ 478.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
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1 ABIN5552208 EIA IHC (p) WB Rabbit Ig Fraction Middle Region Anmelden zum Anzeigen Polyclonal
1 ABIN5552223 EIA IHC (p) WB Rabbit Ig Fraction C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN492351 EIA IP WB Biotin Mouse IgG1 C-Term Anmelden zum Anzeigen 6G11
1 ABIN492382 EIA IF WB Mouse IgG2b AA 1-360, N-Term Anmelden zum Anzeigen AT1A4
1 ABIN492352 EIA WB Biotin Mouse IgG1 N-Term Anmelden zum Anzeigen 6H3
1 ABIN400733 EIA IHC (fro) IHC (p) WB Mouse IgG2a Anmelden zum Anzeigen 4C11C11C4
1 ABIN641241 CA DB EIA IA ISt ELISA WB Rabbit Anmelden zum Anzeigen Polyclonal

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antikörper
Epitop N-Term
(60), (21), (15), (11), (9), (9), (8), (8), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Hund, Human, Maus, Ratte (Rattus)
(352), (147), (118), (50), (30), (19), (14), (11), (10), (9), (8), (7), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1)
Wirt Maus
(169), (167), (16), (8), (3)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser ERK2 Antikörper ist unkonjugiert
(10), (9), (8), (7), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Applikation Enzyme Immunoassay (EIA), Western Blotting (WB)
(326), (157), (94), (83), (82), (63), (52), (26), (22), (9), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-ERK2 Antikörper

Target Details ERK2 Anwendungsinformationen Handhabung Referenzen für anti-ERK2 Antikörper (ABIN126833) Bilder
Spezifität This antibody specifically recognizes the N-terminus of MAP kinase 2 (ERK2).
Produktmerkmale Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2,MAPK2, MAPK1, Extracellular signal-regulated kinase 2
Reinigung Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Bestandteile incl. positive Control
Immunogen Peptide conjugated to KLHEpitope: N-terminus
Klon 6H3
Isotyp IgG1

Target Details ERK2

Produktdetails anti-ERK2 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-ERK2 Antikörper (ABIN126833) Bilder zurück nach oben
Antigen
Andere Bezeichnung ERK2 (MAPK1 Antibody Abstract)
Hintergrund Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK
Molekulargewicht 42 kDa
Gen-ID 5594
UniProt P28482
Forschungsgebiet Stem Cells, Signaling, Inflammation
Pathways MAPK Signalweg, RTK Signalweg, Apoptose, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagie, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Anwendungsinformationen

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Handhabung Referenzen für anti-ERK2 Antikörper (ABIN126833) Bilder zurück nach oben
Applikationshinweise ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll Positive Control Provided. Cell lysate from untreated HepG2Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Anwendungsinformationen Referenzen für anti-ERK2 Antikörper (ABIN126833) Bilder zurück nach oben
Rekonstitution Restore with 1.0 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS containing PEG and Sucrose, 0.09 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Haltbarkeit 12 months

Referenzen für anti-ERK2 Antikörper (ABIN126833)

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Samuels, Karlo, Faruzzi, Pickering, Herrup, Sweatt, Saitta, Landreth: "Deletion of ERK2 mitogen-activated protein kinase identifies its key roles in cortical neurogenesis and cognitive function." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 27, pp. 6983-95, 2008

Bilder

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Anwendungsinformationen Handhabung Referenzen für anti-ERK2 Antikörper (ABIN126833) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (N-Term) antibody (ABIN126833) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (N-Term) antibody