anti-Alle Spezies TUBA1B Antikörper für Western Blotting

Recommended TUBA1B Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tubulin, alpha 1B (TUBA1B) Antikörper
  • K-ALPHA-1
  • k-alpha-1
  • tuba
  • tuba1
  • Tuba2
  • RGD1565476
  • TUBA3
  • TUBAT
  • similar to alpha tubulin
  • alpha tubulin
  • tubulin, alpha 1b
  • tubulin, alpha 1B
  • tubulin, alpha 8
  • RGD1560000
  • LOC100009327
  • TUBA1B
  • tuba1b
  • Tuba1b
  • TUBA8
Epitop
AA 65-97, N-Term
9
8
5
4
3
2
2
1
1
1
Reaktivität
Alle Spezies
48
21
17
7
5
5
5
5
3
3
3
2
2
2
1
1
1
Wirt
Maus
32
22
2
Klonalität
Monoklonal
Konjugat
Dieser TUBA1B Antikörper ist konjugiert mit FITC
3
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
53
29
26
18
17
11
9
7
7
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN93892
297,00 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
2.906104 ABIN301997 ICC IHC (p) WB Biotin Mouse IgG1 AA 65-97, N-Term Anmelden zum Anzeigen TU-01 13
1 ABIN112505 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 Anmelden zum Anzeigen TU-01 10
1 ABIN303511 WB Biotin Mouse IgG1 Anmelden zum Anzeigen TU-01 4
1 ABIN400675 EIA IF IHC (p) IP WB Mouse IgM Anmelden zum Anzeigen TU-16
1 ABIN93894 ICC IHC (p) IP ELISA WB Mouse IgM Anmelden zum Anzeigen TU-16 3
1 ABIN120542 EIA IHC (fro) IF IHC (p) IP WB Mouse IgM Anmelden zum Anzeigen TU-16

General

Antigen Tubulin, alpha 1B (TUBA1B) Antikörper
Epitop AA 65-97, N-Term
(9), (8), (5), (4), (3), (2), (2), (1), (1), (1)
Reaktivität Alle Spezies
(48), (21), (17), (7), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Wirt Maus
(32), (22), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser TUBA1B Antikörper ist konjugiert mit FITC
(3), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(53), (29), (26), (18), (17), (11), (9), (7), (7), (2), (1)
Pubmed 14 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TUBA1B Antikörper

Target Details TUBA1B Anwendungsinformationen Handhabung ProductDetails: References for anti-TUBA1B antibody (ABIN93892) Bilder
Spezifität The antibody TU-01 recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
Produktmerkmale The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Immunogen Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
Klon TU-01
Isotyp IgG1

Target Details TUBA1B

Produktdetails anti-TUBA1B Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-TUBA1B antibody (ABIN93892) Bilder zurück nach oben
Antigen
Andere Bezeichnung TUBA1B (TUBA1B Antibody Abstract)
Hintergrund The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression.Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
Forschungsgebiet Cytoskeleton
Pathways Microtubule Dynamics, M Phase

Anwendungsinformationen

Produktdetails anti-TUBA1B Antikörper Target Details TUBA1B Handhabung ProductDetails: References for anti-TUBA1B antibody (ABIN93892) Bilder zurück nach oben
Applikationshinweise The reagent is designed for direct immunofluorescence analysis on fixed and permeabilized cells. Suggested working dilution is 1:50. It is recommended that the user titrates the reagent for use in the particular testing system.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TUBA1B Antikörper Target Details TUBA1B Anwendungsinformationen ProductDetails: References for anti-TUBA1B antibody (ABIN93892) Bilder zurück nach oben
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handhabung Do not freeze.
Avoid prolonged exposure to light.
Lagerung 4 °C
Informationen zur Lagerung Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

ProductDetails: References for anti-TUBA1B antibody (ABIN93892)

Produktdetails anti-TUBA1B Antikörper Target Details TUBA1B Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Miyajima, Maruyama, Nonomura, Hatakeyama: "TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression." in: Biochemical and biophysical research communications, Vol. 381, Issue 3, pp. 383-7, 2009

Honys, R?nuak, Fecikovua, Jedelskuy, Nebesuarovua, Dobrev, Capkovua: "Cytoskeleton-associated large RNP complexes in tobacco male gametophyte (EPPs) are associated with ribosomes and are involved in protein synthesis, processing, and localization." in: Journal of proteome research, Vol. 8, Issue 4, pp. 2015-31, 2009

Bon, Di Carlo, Folgiero, Avetrani, Lazzari, DOrazi, Brizzi, Sacchi, Soddu, Blandino, Mottolese, Falcioni: "Negative regulation of beta4 integrin transcription by homeodomain-interacting protein kinase 2 and p53 impairs tumor progression." in: Cancer research, Vol. 69, Issue 14, pp. 5978-86, 2009

Produkt verwendet in:

Lukas, Mazna, Valenta, Doubravska, Pospichalova, Vojtechova, Fafilek, Ivanek, Plachy, Novak, Korinek: "Dazap2 modulates transcription driven by the Wnt effector TCF-4." in: Nucleic acids research, Vol. 37, Issue 9, pp. 3007-20, 2009

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004

Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." in: Planta, Vol. 201, Issue 3, pp. 349-58, 1997

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, 1997

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996

Linhartová, Dráber, Dráberová, Viklický: "Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins." in: The Biochemical journal, Vol. 288 ( Pt 3), pp. 919-24, 1993

Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." in: Histochemistry, Vol. 95, Issue 5, pp. 519-24, 1991

Dráber, Dráberová, Linhartová, Viklický: "Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies." in: Journal of cell science, Vol. 92 ( Pt 3), pp. 519-28, 1990

Dráber, Dráberová, Zicconi, Sellitto, Viklický, Cappuccinelli: "Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin." in: European journal of cell biology, Vol. 41, Issue 1, pp. 82-8, 1987

Grimm, Breitling, Little: "Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1." in: Biochimica et biophysica acta, Vol. 914, Issue 1, pp. 83-8, 1987

Viklický, Dráber, Hasek, Bártek: "Production and characterization of a monoclonal antitubulin antibody." in: Cell biology international reports, Vol. 6, Issue 8, pp. 725-31, 1982

Bilder

Produktdetails anti-TUBA1B Antikörper Target Details TUBA1B Anwendungsinformationen Handhabung ProductDetails: References for anti-TUBA1B antibody (ABIN93892) zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Tubulin, alpha 1B (TUBA1B) (AA 65-97), (N-Term) antibody (FITC) (ABIN93892) Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-al...
Immunofluorescence (IF) image for anti-Tubulin, alpha 1B (TUBA1B) (AA 65-97), (N-Term) antibody (FITC) (ABIN93892) Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha...
Immunofluorescence (IF) image for anti-Tubulin, alpha 1B (TUBA1B) (AA 65-97), (N-Term) antibody (FITC) (ABIN93892) Nucleus is stained with DAPI (blue).
Western Blotting (WB) image for anti-Tubulin, alpha 1B (TUBA1B) (AA 65-97), (N-Term) antibody (FITC) (ABIN93892) Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting ex...
Western Blotting (WB) image for anti-Tubulin, alpha 1B (TUBA1B) (AA 65-97), (N-Term) antibody (FITC) (ABIN93892) Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting ex...