anti-Yeast SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) Antikörper für Enzyme Immunoassay

Recommended SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) (SUMO1) Antikörper
  • CG4494
  • Dm0342
  • DmSUMO-1
  • DmSmt3
  • Dmel\\CG4494
  • Dmsmt3
  • SMT3
  • SUMO
  • Smt3
  • Sumo
  • anon-EST:Posey240
  • dSmt3
  • dsmt3
  • l(2)04493
  • l(2)SH0182
  • l(2)SH2 0182
  • sumo
  • DAP1
  • GMP1
  • OFC10
  • PIC1
  • SENP2
  • SMT3C
  • SMT3H3
  • UBL1
  • 4921531N22Rik
  • AI789753
  • AU040360
  • hm:zeh0670
  • wu:fb74c02
  • wu:fd12c02
  • zgc:65934
  • zgc:85634
  • SUMO1
  • dap1
  • gmp1
  • ofc10
  • pic1
  • senp2
  • smt3
  • smt3c
  • sumo-1
  • sumo1
  • ubl1
  • xsumo-1
  • xsumo1
  • ARABIDOPSIS THALIANA SMALL UBIQUITIN-LIKE MODIFIER 1
  • ATSUMO1
  • F10M23.180
  • F10M23_180
  • SMALL UBIQUITIN-LIKE MODIFIER 1
  • SUM1
  • SUMO 1
  • small ubiquitin-like modifier 1
  • pigSUMO1
  • SENTRIN
  • SMTP3
  • SUMO-1
  • Smt3C
  • Ubl1
  • DAP-1
  • Gkap
  • CG4494 gene product from transcript CG4494-RA
  • small ubiquitin-like modifier 1
  • death-associated protein
  • small ubiquitin-like modifier 1 S homeolog
  • small ubiquitin-related protein 1
  • SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
  • SMT3 suppressor of mif two 3 homolog 1 (yeast)
  • death associated protein
  • DLG associated protein 1
  • smt3
  • Sumo1
  • SUMO1
  • Dap
  • sumo1
  • sumo1.S
  • sumo-1
  • DAP
  • Dlgap1
Reaktivität
Yeast
349
153
110
9
8
7
7
6
5
3
3
2
1
1
1
1
1
1
Wirt
Kaninchen
214
128
4
2
2
1
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
17
15
14
10
10
7
6
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
274
135
135
120
111
109
67
53
40
39
10
9
6
2
1
Hersteller
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Bilder

Produktnummer ABIN116958
$ 610.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN400792 EIA WB Mouse IgG1 Anmelden zum Anzeigen 4F2-F5-G2 5

General

Antigen SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) (SUMO1) Antikörper
Reaktivität Yeast
(349), (153), (110), (9), (8), (7), (7), (6), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(214), (128), (4), (2), (2), (1), (1)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(17), (15), (14), (10), (10), (7), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Applikation Enzyme Immunoassay (EIA), Western Blotting (WB)
(274), (135), (135), (120), (111), (109), (67), (53), (40), (39), (10), (9), (6), (2), (1)
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Spezifität Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This purified polyclonal antibody reacts Yeast SUMO by western blot and ELISA.
Reinigung Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis
Immunogen Recombinant yeast SUMO protein.

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung SUMO1 (SUMO1 Antibody Abstract)
Hintergrund Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein: DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. SUMO has been called SMT3 (Yeast), sentrin, PIC1, GMP1 and UBL1. SUMO has been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IκB-α, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO has an apparent molecular weight of ~12 kDa and human SUMO-1 (a 101 amino acid polypeptide) shares 50 % sequence identity with SUMO-2 and SUMO-3 and with yeast SMT3. SUMO and ubiquitin only show about 18 % homology, but both possess a common three-dimensional structure characterized by a tightly packed globular fold with beta-sheets wrapped around an alpha-helix.Synonyms: GAP-modifying protein 1, GMP1, SMT3 homolog 3, SMT3C, SMT3H3, Sentrin, Small ubiquitin-related modifier 1, UBL1, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1
Gen-ID 7341
NCBI Accession NP_001005781
UniProt P63165
Forschungsgebiet Signaling, Proteases, DNA/RNA
Pathways M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikationshinweise Suitable for Western blot (Immunoblotting: 1/1,000) and ELISA (1/1,000-1/5,000). Thisantibody is likely functional in Immunohistochemistry and Immunoprecipitation (nottested). A 12 kDa band corresponding to Yeast SUMO is detected. Most yeast cell lysatescan be used as a positive control without induction or stimulation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Rekonstitution Restore with 0.5 mL of deionized water (or equivalent).
Konzentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing. Dilute only prior to immediate use
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Kahyo, Nishida, Yasuda: "Involvement of PIAS1 in the sumoylation of tumor suppressor p53." in: Molecular cell, Vol. 8, Issue 3, pp. 713-8, 2001

Müller, Hoege, Pyrowolakis, Jentsch: "SUMO, ubiquitin's mysterious cousin." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 3, pp. 202-10, 2001

Hochstrasser: "SP-RING for SUMO: new functions bloom for a ubiquitin-like protein." in: Cell, Vol. 107, Issue 1, pp. 5-8, 2001

Takahashi, Kahyo, Toh-E, Yasuda, Kikuchi: "Yeast Ull1/Siz1 is a novel SUMO1/Smt3 ligase for septin components and functions as an adaptor between conjugating enzyme and substrates." in: The Journal of biological chemistry, Vol. 276, Issue 52, pp. 48973-7, 2001

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
 image for anti-SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) (SUMO1) antibody (ABIN116958) Immunoblot of SUMO-GFP fusion proteins cleaved by insect cell protein extracts. Anti-...