bmMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured)
(1 reference)-
- Proteinspezies
- Human
- Lysatspezies
- Human Cells
- Applikation
- Immunoprecipitation (IP), Western Blotting (WB)
- Verwendungszweck
- Whole cell lysate
- Spezifität
- Non-denatured
- Produktmerkmale
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b mMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured)
Used Tissue: Bone Marrow - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Cell Lysate
- Lysate Fraction
- Whole Cell Lysate
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BIRC3 293T Cell Transient Overexpression Lysate(Denatured)
BIRC3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
APLP2 293T Cell Transient Overexpression Lysate(Denatured)APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
BAG1 293T Cell Transient Overexpression Lysate(Denatured)BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
VNN1 293T Cell Transient Overexpression Lysate(Denatured)VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
Hep2 Whole Cell LysateWB Whole Cell Lysate Cell Lysate HepG2 Cells Reaktivität: Human
NIH/3T3 Whole Cell Lysate (PDGF Stimulated)WB Whole Cell Lysate Cell Lysate 3T3 Cells PDGF Stimulated Reaktivität: Maus
DYKDDDDK Positive Control LysatePC, WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Tag
A431 Whole Cell Lysate (EGF Stimulated)WB Whole Cell Lysate Cell Lysate A431 Cells EGF treated Reaktivität: Human
CD177 (human): 293T LysateCD177 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells
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- Applikationshinweise
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Kommentare
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Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handhabung
- Aliquot to avoid repeated freezing and thawing.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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: "Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 3, pp. 679-85, (2006) (PubMed).
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: "Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 3, pp. 679-85, (2006) (PubMed).
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- Hintergrund
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Full Gene Name:
Synonyms:
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