anti-Human Prostate Specific Antigen Antikörper für Flow Cytometry

Recommended Prostate Specific Antigen Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Prostate Specific Antigen (PSA) Antikörper
  • APS
  • KLK2A1
  • PSA
  • hK3
  • AAP-S
  • MP100
  • KLKB1
  • KLK3
  • Klk1c10l2
  • RSGK-50
  • RSKG-50
  • rGK-3
  • Kal-3
  • Kal3
  • Klk3
  • kallikrein-related peptidase 3
  • aminopeptidase puromycin sensitive
  • kallikrein 3
  • kallikrein B, plasma (Fletcher factor) 1
  • kallikrein 1-related peptidase C3
  • kallikrein B, plasma 1
  • KLK3
  • NPEPPS
  • KLKB1
  • Klk1c3
  • Klkb1
Reaktivität
Human
734
28
9
5
1
1
Wirt
Maus
585
117
25
9
6
Klonalität
Monoklonal
Konjugat
Dieser Prostate Specific Antigen Antikörper ist unkonjugiert
43
31
22
11
11
9
9
7
7
7
7
7
7
7
7
7
7
7
7
5
2
2
2
2
2
2
2
2
2
1
Applikation
Dot Blot (DB), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
444
230
215
146
115
88
87
60
32
26
25
17
14
11
7
7
6
6
5
4
2
2
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN1543584
588,50 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
13.342041 ABIN179726 FACS IHC Mouse IgG1 Anmelden zum Anzeigen LT-3D2 3
13.342041 ABIN3072908 FACS IHC ELISA WB Mouse IgG1 AA 26-251 Anmelden zum Anzeigen 5A11E9 2
13.342041 ABIN1107903 EIA FACS WB Mouse IgG Anmelden zum Anzeigen 8A12 1
13.342041 ABIN4328022 ELISA FACS IHC IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 5A11E9
13.342041 ABIN1583291 FACS IHC ELISA WB Mouse IgG1 AA 26-251 Anmelden zum Anzeigen 5A11E9-5A11E2
13.342041 ABIN4227995 ELISA FACS WB Mouse Anmelden zum Anzeigen 8A12
13.342041 ABIN2983296 FACS IHC ELISA WB Mouse IgG1 AA 26-251 Anmelden zum Anzeigen
13.342041 ABIN2866962 FACS LF IHC ELISA WB Mouse IgG1 kappa Anmelden zum Anzeigen
13.342041 ABIN2866964 FACS LF IHC ELISA WB Mouse IgG1 kappa Anmelden zum Anzeigen
13.342041 ABIN2866963 FACS LF IHC ELISA WB Mouse IgG1 kappa Anmelden zum Anzeigen
1 ABIN492670 EIA FACS IHC (p) WB Mouse IgG1 AA 26-251 Anmelden zum Anzeigen 5A11E2
1 ABIN462157 FACS IHC (p) ELISA WB Mouse IgG1 AA 26-251 Anmelden zum Anzeigen 5A11E2
1 ABIN1500449 FACS ELISA WB Mouse IgG1 Anmelden zum Anzeigen 8A12
1 ABIN2469948 FACS IHC ELISA WB Mouse IgG1 AA 26-251 Anmelden zum Anzeigen 5A11E2
1 ABIN4328020 ELISA FACS IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 5A11E2
1 ABIN336381 FACS IP Lbl IHC ELISA WB Goat Anmelden zum Anzeigen Polyclonal 1
1 ABIN1502669 ELISpot ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 1A7
1 ABIN1502670 ELISpot ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 3E6
1 ABIN1502671 ELISpot ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen A5D5
1 ABIN1502668 ELISpot ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen C3F12

anti-Prostate Specific Antigen Antikörper für Flow Cytometry mit den meisten Publikationen

Ähnliche anti-Prostate Specific Antigen Antikörper

Applikation / Reaktivität Human
Cytometry by Time of Flight (CyTOF) 6 Antikörper
Dot Blot (DB) 8 Antikörper
ELISA 438 Antikörper
ELISA (Capture) 4 Antikörper
ELISA (Detection) 2 Antikörper
ELISpot 11 Antikörper
Enzyme Immunoassay (EIA) 32 Antikörper
Flow Cytometry (FACS) 147 Antikörper
Immunoassay (IA) 7 Antikörper
Immunochromatography (IC) 6 Antikörper
Immunocytochemistry (ICC) 58 Antikörper
Immunodiffusion (ID) 1 Antikörper
Immunofluorescence (IF) 87 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 1 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antikörper
Immunohistochemistry (IHC) 113 Antikörper

General

Antigen Prostate Specific Antigen (PSA) Antikörper
Reaktivität Human
(734), (28), (9), (5), (1), (1)
Wirt Maus
(585), (117), (25), (9), (6)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Prostate Specific Antigen Antikörper ist unkonjugiert
(43), (31), (22), (11), (11), (9), (9), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Applikation Dot Blot (DB), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(444), (230), (215), (146), (115), (88), (87), (60), (32), (26), (25), (17), (14), (11), (7), (7), (6), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Prostate Specific Antigen Antikörper

Target Details Prostate Specific Antigen Anwendungsinformationen Handhabung Bilder
Reinigung Protein A affinity column
Immunogen Native PSA Antigen
Klon A5D5-1
Isotyp IgG1

Target Details Prostate Specific Antigen

Produktdetails anti-Prostate Specific Antigen Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung PSA (PSA Antibody Abstract)
Forschungsgebiet Cancer, Cell/Tissue Markers
Pathways Komplementsystem

Anwendungsinformationen

Produktdetails anti-Prostate Specific Antigen Antikörper Target Details Prostate Specific Antigen Handhabung Bilder zurück nach oben
Kommentare

Other Applications have not been tested.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Prostate Specific Antigen Antikörper Target Details Prostate Specific Antigen Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1.8 mg/mL
Buffer PBS, pH 7.4, 0.09% sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
'Independent Validation' Siegel
Chargennummer FAPS-A5D5-0003
Validierte Anwendung Flow Cytometry
Positivkontrolle LNCaP cells
Negativkontrolle - DU145 cells - Jurkat cells
Bewertung Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: LNCaP prostate tumor cell line
  • Negative control: DU145 prostate tumor cell line, Jurkat T cell leukemia cell line
  • Isotype control: cells treated with mouse IgG1 instead of the primary antibody to confirm that primary antibody binding is specific.
Protokoll
  • Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
  • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
  • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
  • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
  • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
  • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
  • 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
  • Cells were washed twice with PBS/BSA/Tween 20
  • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
  • Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
  • Cells were washed twice with PBS/BSA/Tween 20
  • Cells were resuspended in 0.3 mL PBS/BSA
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
Anmerkungen None
Validierungsbilder
Flow Cytometry validation image for anti-Prostate Specific Antigen (PSA) antibody (ABIN1543584) Figure 1: LNCaP cells stained with anti-PSA (blue) or with isotype control (red).
Flow Cytometry validation image for anti-Prostate Specific Antigen (PSA) antibody (ABIN1543584) Figure 2: DU145 cells stained with anti-PSA (blue) or with isotype control (red).
Flow Cytometry validation image for anti-Prostate Specific Antigen (PSA) antibody (ABIN1543584) Figure 3: Jurkat cells stained with anti-PSA (blue) or with isotype control (red).