Human Kallikrein 13 ELISA Kit

Recommended Kallikrein 13 Product (geliefert von: Anmelden zum Anzeigen )

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Kallikrein-Related Peptidase 13 (KLK13) ELISA Kits
  • Egfbp-2
  • mGk-13
  • KLK-L4
  • KLKL4
  • kallikrein related-peptidase 13
  • kallikrein-13-like
  • kallikrein-related peptidase 13
  • Klk13
  • LOC540297
  • KLK13
Epitop
AA 17-277
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
8
5
Methodentyp
Sandwich ELISA
Detektionsbereich
156-10.000 pg/mL
Untere Nachweisgrenze
156 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Bilder

Produktnummer ABIN2859316
475,62 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
1 ABIN1874354 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN1570914 Sandwich ELISA Biological Fluids, Cell Lysate, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN842247 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 12.5-800 ng/mL 12.5 ng/mL Anmelden zum Anzeigen
1 ABIN4883529 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell and Tissue Lysate Anmelden zum Anzeigen
1 ABIN5499274 Sandwich ELISA Recombinant Protein 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
1 ABIN2535750 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN4970604 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
1 ABIN2043839 Sandwich ELISA 0.15-10 ng/mL 0.15 ng/mL Anmelden zum Anzeigen

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General

Antigen Kallikrein-Related Peptidase 13 (KLK13) ELISA Kits
Epitop AA 17-277
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(8), (5)
Methodentyp Sandwich ELISA
Detektionsbereich 156-10.000 pg/mL
Untere Nachweisgrenze 156 pg/mL
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails Kallikrein 13 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK13
Marke PicoKine™
Proben Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität NSO, G17-Q277
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: G17-Q277

Antigendetails

Produktdetails Kallikrein 13 ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
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Andere Bezeichnung KLK13 (KLK13 ELISA Kit Abstract)
Hintergrund

Background: Kallikrein-13 is a protein that in humans is encoded by the KLK13 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. By genomic sequence analysis, KLK13 gene is mapped in a 300-kb region on chromosome 19q13.3-q13.4. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components. However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies.

Synonyms: Kallikrein-13,3.4.21.-,Kallikrein-like protein 4,KLK-L4,KLK13,KLKL4,

Full Gene Name: Kallikrein-13

Cellular Localisation: Secreted.
Gen-ID 26085
UniProt Q9UKR3
Pathways Komplementsystem

Anwendungsinformationen

Produktdetails Kallikrein 13 ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Tissue Specificity: Expressed in prostate, breast, testis and salivary gland.

Plattentyp Pre-coated
Protokoll human KLK13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK13 has been precoated onto 96-well plates. Standards (NSO,G17-Q277) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK13 amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KLK13 standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 1661, Standard deviation: 83.05, CV(%): 5.0
  • Sample 2: n=16, Mean(pg/ml): 3572, Standard deviation: 196.46, CV(%): 5.5
  • Sample 3: n=16, Mean(pg/ml): 6985, Standard deviation: 405.13, CV(%): 5.8,
  • Sample 1: n=24, Mean(pg/ml): 1369, Standard deviation: 71.18, CV(%): 5.2
  • Sample 2: n=24, Mean(pg/ml): 4425, Standard deviation: 269.92, CV(%): 6.1
  • Sample 3: n=24, Mean(pg/ml): 6594, Standard deviation: 422.01, CV(%): 6.4
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

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Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

Bilder

Produktdetails Kallikrein 13 ELISA Kit Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
ELISA image for Kallikrein 13 ELISA Kit (Kallikrein-Related Peptidase 13) (ABIN2859316) Human KLK13 PicoKine ELISA Kit standard curve