Human FCN2 ELISA Kit

Recommended FCN2 Product (geliefert von: Anmelden zum Anzeigen )

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Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2) ELISA Kits
  • EBP-37
  • FCNL
  • P35
  • ficolin-2
  • FCN2
  • FCNB
  • Fcn2
  • ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
  • ficolin (collagen/fibrinogen domain containing) 1
  • ficolin B
  • FCN2
  • FCN1
  • Fcnb
Epitop
AA 26-313
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
14
2
2
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
0.78-50 ng/mL
Untere Nachweisgrenze
0.78 ng/mL
Applikation
ELISA
Optionen
Hersteller
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Produktnummer ABIN1889409
$ 369.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.090268 ABIN814818 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.25-16 ng/mL 0.25 ng/mL Anmelden zum Anzeigen
10.090268 ABIN415093 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 0.78-50 ng/mL 0.78 ng/mL Anmelden zum Anzeigen
10.090268 ABIN1028659 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
10.090268 ABIN2708213 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 780-50000 pg/mL 780 pg/mL Anmelden zum Anzeigen
1 ABIN5594738 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN4882826 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 0.123-30 ng/mL 0.123 ng/mL Anmelden zum Anzeigen
1 ABIN5654345 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.781 ng/mL - 50 ng/mL 0.781 ng/mL Anmelden zum Anzeigen
1 ABIN5524772 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN2684244 Sandwich ELISA Plasma (heparin), Cell Culture Supernatant, Serum 0.78-50 ng/mL 0.78 ng/mL Anmelden zum Anzeigen
1 ABIN2533479 Sandwich ELISA Serum, Plasma, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Anmelden zum Anzeigen
1 ABIN4969203 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN455125 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN2947542 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN5073225 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Anmelden zum Anzeigen

General

Antigen Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2) ELISA Kits
Epitop AA 26-313
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(14), (2), (2), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 0.78-50 ng/mL
Untere Nachweisgrenze 0.78 ng/mL
Applikation ELISA
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails FCN2 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Referenzen für FCN2 Kit (ABIN1889409) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Ficolin-2
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin)
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: NSO
Immunogen sequence: L26-A313
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <20pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: L26-A313

Antigendetails

Produktdetails FCN2 ELISA Kit Anwendungsinformationen Handhabung Referenzen für FCN2 Kit (ABIN1889409) Bilder zurück nach oben
Antigen
Andere Bezeichnung FCN2 (FCN2 ELISA Kit Abstract)
Hintergrund

Protein Function: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. .

Background: Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in theficolin-2 serum concentration.

Synonyms: Ficolin-2,37 kDa elastin-binding protein,Collagen/fibrinogen domain-containing protein 2,EBP-37,Ficolin-B,Ficolin-beta,Hucolin,L-ficolin,Serum lectin p35,FCN2,FCNL,

Full Gene Name: Ficolin-2

Cellular Localisation: Secreted.
Gen-ID 2220
UniProt Q15485
Pathways Komplementsystem

Anwendungsinformationen

Produktdetails FCN2 ELISA Kit Antigendetails Handhabung Referenzen für FCN2 Kit (ABIN1889409) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Belongs to the ficolin lectin family.

Tissue Specificity: Expressed by the liver and secreted in plasma.

Plattentyp Pre-coated
Protokoll human Ficolin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-2 has been precoated onto 96-well plates. Standards(NSO, L26-A313) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Ficolin-2 amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Ficolin-2 standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(ng/ml): 4.78, Standard deviation: 0.186, CV(%): 3.9
  • Sample 2: n=16, Mean(ng/ml): 12.7, Standard deviation: 0.648, CV(%): 5.1
  • Sample 3: n=16, Mean(ng/ml): 24.1, Standard deviation: 1.11, CV(%): 4.6,
  • Sample 1: n=24, Mean(ng/ml): 5.14, Standard deviation: 0.298, CV(%): 5.8
  • Sample 2: n=24, Mean(ng/ml): 13.2, Standard deviation: 0.99, CV(%): 7.5
  • Sample 3: n=24, Mean(ng/ml): 25.7, Standard deviation: 1.62, CV(%): 6.3
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails FCN2 ELISA Kit Antigendetails Anwendungsinformationen Referenzen für FCN2 Kit (ABIN1889409) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

Referenzen für FCN2 Kit (ABIN1889409)

Produktdetails FCN2 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Hummelshoj, Munthe-Fog, Madsen, Fujita, Matsushita, Garred: "Polymorphisms in the FCN2 gene determine serum variation and function of Ficolin-2." in: Human molecular genetics, Vol. 14, Issue 12, pp. 1651-8, 2005

Matsushita, Endo, Taira, Sato, Fujita, Ichikawa, Nakata, Mizuochi: "A novel human serum lectin with collagen- and fibrinogen-like domains that functions as an opsonin." in: The Journal of biological chemistry, Vol. 271, Issue 5, pp. 2448-54, 1996

Bilder

Produktdetails FCN2 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für FCN2 Kit (ABIN1889409) zurück nach oben
Bilder des Herstellers
ELISA image for Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2) ELISA Kit (ABIN1889409) Human Ficolin-2 PicoKine ELISA Kit standard curve